| RAPD分子标记在蜜蜂遗传育种中的应用 |
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| 引用本文： | 苏松坤,陈盛禄.RAPD分子标记在蜜蜂遗传育种中的应用[J].福建农林大学学报(自然科学版),2002,31(2):251-254. |
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| 作者姓名： | 苏松坤 陈盛禄 |
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| 作者单位： | 浙江大学动物科学学院,浙江,杭州,310029 |
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| 基金项目： | 浙江省自然科学基金资助项目 (3 99479) |
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| 摘 要： | RAPD分子标记在蜜蜂遗传育种中得到较广泛应用 .利用 RAPD分子遗传标记 ,估测蜜蜂性别决定位点 (x)与低幼虫存活率位点的标记序列位置 (STS)之间的遗传距离为 1 .6c M;构建了蜜蜂 (Apismellifera)遗传连锁图 ,确定了蜜蜂性基因位点 (x)、黑色体色基因位点 (blk)、苹果酸脱氢酶 (Mdh-1 )位点分别位于第 3、6、1 8个连锁群 ;确定了影响蜜蜂采粉、报警信息素水平、螫刺行为和体长等数量性状的基因座位 ;筛选出 5种引物 ,这些引物所扩增的 RAPD标记可作为鉴别欧洲蜜蜂和非洲蜜蜂 (A. mellifera L.)的分子标记 .蜜蜂高产性状 RAPD标记的研究已取得一定进展
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| 关 键 词： | 蜜蜂 RAPD 遗传 育种 |
| 文章编号： | 1006-7817(2002)02-0251-04 |
| 修稿时间： | 2001年10月8日 |
Application of RAPD molecular markers in the heredity and breeding of honey bees |
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| SU Song kun,CHEN Sheng lu.Application of RAPD molecular markers in the heredity and breeding of honey bees[J].Journal of Fujian Agricultural and Forestry University,2002,31(2):251-254. |
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| Authors: | SU Song kun CHEN Sheng lu |
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| Abstract: | With the molecular markers of random amplified polymorphic DNA (RAPD), the genetic distance between sex determination locus ( x ) of honey bee and the sequence tagged site (STS) for low brood viability locus was estimated at 1.6 cM. A linkage map was constructed for honey bee( Apis mellifera ) based on RAPD markers. The x locus for sex determination and genes for black body color and malate dehydrogenase were mapped on linkage group Ⅲ, Ⅵ, ⅩⅧ, respectively. The quantitative trait loci influencing the amount of pollen stored in combs of honey bee colonies, honey bee alarm pheromone levels, honey bee stinging behavior and body size were identified, respectively. Five primers were selected for amplifying different RAPD markers to distinguish African and European honey bees ( A. mellifera L.). The research of RAPD markers for high yield traits of honey bees has made some progress. |
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| Keywords: | honey bee RAPD heredity breeding |
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