| 玉米转Bt基因后代的抗虫性鉴定及其遗传分析 |
| |
| 引用本文： | 朱卫红,王庆东,刘宗华,汤继华,赵广远,胡彦民,靳秋生.玉米转Bt基因后代的抗虫性鉴定及其遗传分析[J].河南农业大学学报,2004,38(2):135-138. |
| |
| 作者姓名： | 朱卫红 王庆东 刘宗华 汤继华 赵广远 胡彦民 靳秋生 |
| |
| 作者单位： | 1. 河南农业大学,河南,郑州,450002
2. 焦作市农科所,河南,焦作,454151 |
| |
| 基金项目： | 河南省自然科学基金资助项目(2003210079) |
| |
| 摘 要： | 采用田间接虫鉴定与室内PCR扩增，对玉米4个遗传背景，2个不同回交世代转基因材料进行了抗虫性鉴定．结果表明：回交3代、4代转基因材料平均抗虫性与其阴性对照相比达到了显著或极显著水平，外源Bt基因在回交后代中符合孟德尔定律1:1的分离比例．在4个遗传背景共98株含Bt基因的单株中，田间抗虫性表现高抗73株，抗5株，中抗7株，感2株，高感11株，抗虫株率占86．7％．
|
| 关 键 词： | 玉米 转Bt基因 抗虫性 鉴定 遗传分析 孟德尔定律 |
| 文章编号： | 1000-2340(2004)02-0135-04 |
Identification of insect resistance and inheritance analysis of transgenic Bt gene in maize |
| |
| ZHU Wei-hong,WANG Qing-dong,LIU Zong-hua,et al.Identification of insect resistance and inheritance analysis of transgenic Bt gene in maize[J].Journal of Henan Agricultural University,2004,38(2):135-138. |
| |
| Authors: | ZHU Wei-hong WANG Qing-dong LIU Zong-hua |
| |
| Abstract: | The identification of insect resistance has been made on the four backcross materials by inoculating insect eggs into leaf spindle in the field and indoor PCR.The result shows that the difference of mean insect resistance of BC_3 and BC_4 is in a remarkable or much more outstanding standard.The result of PCR shows that Mendelian segregation was observed in BC_3 and BC_4.In 98 individuals,identified with external Bt toxic protein gene, the numbers of individuals of high resistance(HR), resistance(R),medial resistance(MR), susceptivity(S), high susceptivity(HS) are respectively 73, 5, 7,2 and 11.The ratio of insect resistant plants is 86.7%. |
| |
| Keywords: | maize transgenic corn borer resistance PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
