Validation of an immunohistochemical assay for bovine cysticercosis, with comparison to a standard histological method |
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Authors: | Scandrett W Brad Haines Deborah M Parker Sarah E Robinson Yves Forbes Lorry B Brandt Jef Geerts Stanny Dorny Pierre Gajadhar Alvin A |
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Affiliation: | Centre for Food-borne and Animal Parasitology, Canadian Food Inspection Agency, Saskatoon Laboratory, 116 Veterinary Road, Saskatoon, Saskatchewan S7N 2R3, Canada. brad.scandrett@inspection.gc.ca |
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Abstract: | The larval stage (syn Cysticercus bovis) of the human tapeworm Taenia saginata causes cysticercosis in cattle, which has both aesthetic and food safety implications to consumers of beef. A monoclonal antibody-based immunohistochemical (IHC) assay developed to improve postmortem diagnosis of this parasite and a standard histological method were assessed to determine their fitness for intended use. Sections from 169 known-positive specimens of T. saginata from experimentally or naturally infected cattle, and from 30 known-negative specimens and lesions of various etiologies from non-infected cattle, were tested. The IHC assay identified significantly more known positive bovine cysticerci than the histological method (91.7% and 38.5%, respectively). Positive IHC staining occurred on sections from other cestode species, but should not affect the diagnostic specificity of this assay for bovine cysticercosis, due to the different host and/or tissue preferences amongst these parasites. Use of the IHC assay should improve the reliability of diagnosing lesions caused by degenerated cysticerci, facilitating more effective and efficient control of bovine cysticercosis. |
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