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| 东莨菪素对朱砂叶螨的毒力及杀螨机理初探 | |
| 引用本文： | 梁为,白雪娜,马兰青,师光禄,王有年.东莨菪素对朱砂叶螨的毒力及杀螨机理初探[J].广东农业科学,2011,38(8):68-71. |
| 作者姓名： | 梁为 白雪娜 马兰青 师光禄 王有年 |
| 作者单位： | 1. 北京林业大学林学院,北京,100083 2. 农业部都市农业(北方)重点开放实验室,北京 100083;北京农学院植物科学技术学院,北京 102206 3. 农业部都市农业(北方)重点开放实验室,北京,100083 |
| 基金项目： | 国家自然科学基金(30872029); 北京市自然科学基金重点项目(6071001);北京市自然科学基金(6092007); 北京市教委重点项目(KZ201010020016);北京市教委平台建设项目; 北京市属高校人才强教深化计划项目(PHR20090516,PHR200906134) |
| 摘 要： | 采用玻片浸渍法测定东莨菪素对朱砂叶螨的触杀毒力,观察东莨菪素对朱砂叶螨的致毒症状,并测定其对朱砂叶螨神经系统靶标酶(乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶)活性的影响。结果表明,东莨菪素对朱砂叶螨24 h的触杀LC50值为1.24 mg/mL;中毒试螨表现出神经毒剂的致毒症状,如兴奋、痉挛等。在活体和离体试验中,东莨菪素均显著抑制试螨乙酰胆碱酯酶、单胺氧化酶、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性。研究还发现东莨菪素会引起试螨乙酰胆碱含量增加,可能是东莨菪素显著抑制试螨乙酰胆碱酯酶活性引起。据此推测东莨菪素对朱砂叶螨可能有神经毒性。 |
| 关 键 词： | 东莨菪素 朱砂叶螨 触杀毒力 酶活性 |
Preliminary study on scopoletin toxicity to Tetranychus cinnabarinus and its acaricidal mechanism |
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| LIANG Wei,BAI Xue-na,MA Lan-qing,SHI Guang-lu,WANG You-nian.Preliminary study on scopoletin toxicity to Tetranychus cinnabarinus and its acaricidal mechanism[J].Guangdong Agricultural Sciences,2011,38(8):68-71. | |
| Authors: | LIANG Wei BAI Xue-na MA Lan-qing SHI Guang-lu WANG You-nian |
| Institution: | LIANG Wei1,BAI Xue-na2,3,MA Lan-qing2,SHI Guang-lu2,WANG You-nian2,3(1.College of Forestry of Beijing Forestry University,Beijing 100083,China,2.Key Laboratory of Urban Agriculture(North) Ministry of Agriculture Beijing 100083,China 3.Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China) |
| Abstract: | In this study,the contact toxicity of scopoletin to Tetranychus cinnabarinus was tested using slide dip method,and dynamic toxicosis symptoms of scopoletin-exposed mites were also detailedly observed.The results showed that the contact toxicity LC50 value of scopoletin to T.cinnabarinus was 1.24 mg/mL at 24th h post-treatment.Some typical neurotoxic symptoms such as excitement and convulsions were observed in scopoletin-exposed mites.Subsequently,the changes of the in vitro and ex vitro activities of AChE,M... |
| Keywords: | scopoletin Tetranychus cinnabarinus acaricidal activity enzyme activity |
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