| 紫外线诱变纤维素酶高产菌株的筛选及其酶活力 |
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| 引用本文： | 张君胜,张力,张尧. 紫外线诱变纤维素酶高产菌株的筛选及其酶活力[J]. 贵州农业科学, 2011, 0(10): 125-127 |
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| 作者姓名： | 张君胜 张力 张尧 |
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| 作者单位： | 江苏畜牧兽医职业技术学院; |
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| 基金项目： | 江苏畜牧兽医职业技术学院项目“高活性纤维素酶菌株的筛选”(200825) |
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| 摘 要： | 为筛选产纤维素酶酶活力高的菌株应用于秸秆饲料发酵,利用紫外线诱变对1株产纤维素酶绿色木霉菌进行了试验研究.结果表明:筛选出1株纤维素酶产酶量高且性状稳定的高产菌株ZJUV18,其 发酵72h纤维素酶滤纸酶活达68.07 U/g,较原始菌株提高4.45倍.
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| 关 键 词： | 纤维素酶 诱变 紫外线 酶活力 |
Mutation Screening of High Cellulase-producing Strains by Ultraviolet and Its Enzyme Activity |
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| ZHANG Jun-sheng,ZHANG Li,ZHANG Yao. Mutation Screening of High Cellulase-producing Strains by Ultraviolet and Its Enzyme Activity[J]. Guizhou Agricultural Sciences, 2011, 0(10): 125-127 |
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| Authors: | ZHANG Jun-sheng ZHANG Li ZHANG Yao |
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| Affiliation: | ZHANG Jun-sheng,ZHANG Li,ZHANG Yao(Jiangsu Animal Husbandry and Veterinary College,Taizhou,Jiangsu 225300,China) |
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| Abstract: | A Trichoderma viride strain was treated with ultraviolet to screen out a high cellulase-producing strain and apply in straw feed fermentation.The results showed that a high cellulase-producing strain with high cellulose yield and stable characteristics,which was named ZJUV18,was screened out.The FPA could be up to 68.07 U/g after fermented for 72 hours,which was 4.45 times the activity of the original strain. |
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| Keywords: | cellulase mutagenesis ultraviolet enzyme activity |
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