| 小头飞虱属2个近缘种的ITS分析 |
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| 引用本文： | 侯晓晖,陈祥盛. 小头飞虱属2个近缘种的ITS分析[J]. 贵州农业科学, 2011, 0(11) |
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| 作者姓名： | 侯晓晖 陈祥盛 |
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| 作者单位： | 贵州大学昆虫研究所/贵州山地农业病虫害重点实验室;遵义医学院; |
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| 基金项目： | 国家自然科学基金项目“中国飞虱科系统发育及生物地理学研究”(30100015),“西南地区害竹头喙亚目昆虫物种多样性研究”(30560020),“中国竹子飞虱区系分类、DNA条形码及多媒体鉴定系统研究”(31160163); 贵州省优秀青年科技人才基金项目“中国飞虱科物种多样性研究”[黔科合人字(2005)0520]; 贵州省优秀科技教育人才省长资金“贵州害竹叶蝉和飞虱种类详查”[黔省专合字(2005)357]; 贵州省国际科技合作项目“中加葡萄植原体病害媒介昆虫的鉴定与筛查”[黔科合外G字(2010)7005] |
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| 摘 要： | 为了更准确地鉴别飞虱科近缘种类,采用DNA测序的方法获得小头飞虱属2个近缘种类的ITS1部分序列,并对其进行了分子鉴定。结果表明,黄小头飞虱和四刺小头飞虱ITS1序列平均长度分别为802bp和1 044bp,可初步区分二者;二者遗传距离在种内为0.005～0.035,种间为2.725～2.833,差异显著;系统发育重建显示2个近缘种类各自聚为1支,二者互为姐妹群。研究结果初步证实,通过对ITS1序列信息、遗传距离和系统发育分析等研究,可以准确鉴定飞虱科的近缘种类,进而解决形态学分类时而无法准确鉴定等问题。
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| 关 键 词： | 飞虱 近缘种 小头飞虱属 分子鉴定 ITS1 |
ITS Analysis of Malaxella(Hemiptera:Delphacidae) Based on Internal Transcribed Spacer-1 |
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| HOU Xiao-hui,,CHEN Xiang-sheng. ITS Analysis of Malaxella(Hemiptera:Delphacidae) Based on Internal Transcribed Spacer-1[J]. Guizhou Agricultural Sciences, 2011, 0(11) |
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| Authors: | HOU Xiao-hui CHEN Xiang-sheng |
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| Affiliation: | HOU Xiao-hui1,2,CHEN Xiang-sheng1* (1.Institute of Entomology,Guizhou University,Guizhou Key Laboratory for Plant Pest Management of Mountainous Region,Guiyang,Guizhou 550025,2.Zunyi Medical College,Zunyi,Guizhou 563003,China) |
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| Abstract: | Molecular identification of two related species of bamboo-feeding planthopper genus Malaxella,M.flava and M.tetracantha,was studied based on internal transcribed spacer-1 to identify the related species of Delphacidae.The results showed the sequences length of M.flava and M.tetracantha were 802 and 1 044 respectively,genetic distance of interspecies and intraspecies were obviously different,both category were sister group in phylogenetic analysis.So accuracy identification was possible through the research ... |
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| Keywords: | Delphacidae related species Malaxella molecular identification ITS1 |
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