二氧化硫对玉米细胞SOD活性的影响

选择鲁单981和掖单2号玉米杂交种为实验材料,采用简易自制密闭式熏气装置进行二氧化硫(SO2)短时处理,通过酶活性测定、同工酶电泳及RT-PCR等检测,研究SO2对玉米超氧化物歧化酶(SOD)活性的影响。结果表明:用60mg/m3SO2熏气处理敏感玉米品种鲁单981和不敏感玉米品种掖单2号,SO2对玉米SOD活性没有显著的影响。玉米体内高水平的SOD活性可能与玉米对SO2的抗性有关。酶活性测定、同工酶电泳及RT-PCR分析取得了一致的结果。

Superoxide Dismutase Activity Response to Sulfur Dioxide in Zea mays L.

Sulfur dioxide(SO₂) content in the air is tending higher with the industrial development.Sulfur dioxide pollution causes environment deterioration and acid rain,which bring about severe harmfulness to plant growth and development.The present study was conducted to provide primary result in the area of plant resistance.In a simple self-made obturator (168 cm×65 cm×63 cm),maize seedling was treated with 60 mg/m³ SO₂ for 2 h.The SOD activity of treated leaf was measured at 0,6,12,24,48,and 72 h after treatment,respectively.The SOD isozymes and SOD gene were analyzed by PAGE and RT-PCR,respectively.The results showed that the SOD activity of leaf response to SO₂ no significantly among maize cultivars.However,the SOD activity of control(no SO₂ treatment) leaf was higher at all times.The analysis of isoenzymes and RT-PCR also received the same results,indicating that SOD might play an important role in the interaction of SO₂ and maize.