| 转基因水稻抗坏血酸-谷胱甘肽循环系统对锌镉互作的应答 |
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| 引用本文： | 胡凡,徐忠俊,韩明明,王凯,赵凤云.转基因水稻抗坏血酸-谷胱甘肽循环系统对锌镉互作的应答[J].种子,2010,29(8). |
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| 作者姓名： | 胡凡 徐忠俊 韩明明 王凯 赵凤云 |
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| 作者单位： | 山东理工大学生命科学学院,山东,淄博,255049 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 实验分析了转GST和CAT 1双抗氧化酶基因水稻和非转基因水稻(Oryza sativa L.中花11号)抗坏血酸-谷胱甘肽循环系统在锌、镉及锌镉复合处理条件下的变化。结果显示,在单一镉胁迫下,转基因水稻(T)地上部分的CAT和GST活性都比非转基因水稻(N)的高,而前者的DHAR活性和GSH氧还状态则低于后者的;T根系CAT、APX和GR的活性也高于N的,但其GST、SOD和DHAR的活性则低于N的。单一锌处理时,T地上部分的CAT、GST、DHAR、APX和GR活性及ASA氧还状态都比N的高;其根系CAT和APX的活性也高于N的,但GSH氧还状态则低于后者的。锌和镉复合处理时,T地上部分的CAT和GR活性都比N的高,其SOD和DHAR活性及ASA和GSH氧还状态却低于后者的;T根系的CAT、GST和APX活性都比N的高,而前者的SOD、GR和DHAR活性及GSH氧还状态则比后者的低。转基因水稻和非转基因水稻之间抗坏血酸-谷胱甘肽循环系统变化的差异,可能是外源基因和内源功能相关组分协同作用的结果。
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| 关 键 词： | 水稻抗坏血酸-谷胱甘肽循环系统 镉胁迫 锌镉互作 |
Response of the Ascorbate-glutathione Cycle to Combined Cadmium and Zinc in Transgenic Rice |
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| HU Fan,XU Zhong-jun,HAN Ming-ming,WANG Kai,ZHAO Feng-yun.Response of the Ascorbate-glutathione Cycle to Combined Cadmium and Zinc in Transgenic Rice[J].Seed,2010,29(8). |
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| Authors: | HU Fan XU Zhong-jun HAN Ming-ming WANG Kai ZHAO Feng-yun |
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| Institution: | HU Fan,XU Zhong-jun,HAN Ming-ming,WANG Kai,ZHAO Feng-yun(College of Life Sciences,Shandong University of Technology,Zibo Shandong 255049,Province,China) |
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| Abstract: | An analysis of the change of ascorbate-glutathione cycle in single Cd or Zn and combined Cd and Zn treated transgenic and non-transgenic rice(Oryza sativa L.NO.11) was conducted.The results showed that CAT and GST activity in overground part of transgenetic rice(T) was higher than that of non-transgenetic rice(N) treated with single Cd.but DHAR activity and GSH redox of the former were lower than that of the latter.And the activity of CAT,APS and GR in shoot of T were higher than that of N,but the activity ... |
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| Keywords: | rice ascorbate-glutathione cycle cadmium stress combined cadmium and zinc |
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