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水蔓菁组织培养及植株再生研究
引用本文:刘帆,汤福义,田孟良,乔晓,官宇. 水蔓菁组织培养及植株再生研究[J]. 四川农业大学学报, 2007, 25(2): 117-120
作者姓名:刘帆  汤福义  田孟良  乔晓  官宇
作者单位:四川农业大学,农学院,四川,雅安,625014;四川农业大学,林学园艺学院,四川,雅安,625014
基金项目:四川农业大学校科研和教改项目
摘    要:采用组织培养方法,设置不同激素配比的培养基对水蔓菁(Veronica linariifoliasubsp.dilatata)不同外植体叶片、茎尖及茎段进了行研究。结果表明MS+6-BA 2.0 mg/L+NAA 0.5 mg/L诱导愈伤组织效果最佳,其中以茎尖的诱导效果优于其他外植体;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L诱导愈伤组织丛生芽的分化效果最佳;1/2 MS+IBA 0.5 mg/L诱导丛生芽的生根效果最佳。

关 键 词:水蔓菁  组织培养  植株再生
文章编号:1000-2650(2007)02-0117-04
修稿时间:2007-03-30

Tissue Culture and Plantlet Regeneration of Veronica linariifolia subsp.dilatata
LIU Fan,TANG Fu-yi,TIAN Meng-liang,QIAO Xiao,GUAN Yu. Tissue Culture and Plantlet Regeneration of Veronica linariifolia subsp.dilatata[J]. Journal of Sichuan Agricultural University, 2007, 25(2): 117-120
Authors:LIU Fan  TANG Fu-yi  TIAN Meng-liang  QIAO Xiao  GUAN Yu
Affiliation:1. College of Agriculture; 2. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Siehuan, China
Abstract:To establish a high efficiency plant regeneration system of Veronica linariifolia.,callus,rosette buds,and roots were induced by different combinations of hormone from leaves,stem-tips and stems of Veronica linariifolia.The results showed that the medium of MS+6BA 2.0mg/L+NAA 0.5mg/L was the best for callus induction,the medium of MS+6-BA 2.0mg/L+NAA 0.05mg/L was the best for bud differentiation,and the medium of 1/2 MS+IBA 0.5mg/L was the best e for rooting.
Keywords:Veronica linariifolia   tissue culture   plant regeneration
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