| 高效液相色谱法测定麦芽糖含量 |
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| 引用本文： | 张雪荣,池利民,李素霞. 高效液相色谱法测定麦芽糖含量[J]. 安徽农业科学, 2009, 37(24): 11343-11344 |
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| 作者姓名： | 张雪荣 池利民 李素霞 |
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| 作者单位： | 河北化工医药职业技术学院,河北石家庄,050026;河北化工医药职业技术学院,河北石家庄,050026;河北化工医药职业技术学院,河北石家庄,050026 |
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| 摘 要： | [目的]建立麦芽糖的含量测定方法；[方法]采用高效液相色谱法（HPLC），色谱柱为Alltima氨基2（250mm×4．6mm，5μm），流动相为乙腈-水（62：38），流速为1．3ml／min。[结果]在选定的试验条件下，Alhima氨基柱的理论塔板数为2855，麦芽糖峰与杂质峰的分离度为6．09，精密度试验的RSD为0．90％（n=6）。[结论]该法测定麦芽糖含量具有简捷、实用、准确的特点。
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| 关 键 词： | HPLC 麦芽糖 含量测定 |
Determination of Maltose Contents by HPLC |
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| ZHANG Xue-rong et al. Determination of Maltose Contents by HPLC[J]. Journal of Anhui Agricultural Sciences, 2009, 37(24): 11343-11344 |
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| Authors: | ZHANG Xue-rong et al |
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| Affiliation: | ZHANG Xue-rong et al (Hebei Chemical , Pharmaceutical College,Shijiazhuang,Hebei 050026) |
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| Abstract: | [Objective] The determination method of Maltose contents were established.[Method] HPLC method was used,chromatographic column was the Alltima amido-column(250 mm ×4.6 mm,5 μm),mobile phase was acetonitrile-water(62∶38),flowing rate of 1.3 ml/min.[Result] Under the selected tested conditions,theoretical plate number of the Alltima amido-column was 2 855,the separation degree of Maltose and impurity peak were 6.09,RSD was 0.90%(n=6).[Conclusion] The method was proved to be simple,practical and accurate. |
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| Keywords: | HPLC |
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