Isotopic evidence of significant assimilation of atmospheric-derived nitrogen fixed by Azospirillum brasilense co-inoculated with phosphate-solubilising Pantoea dispersa in pepper seedling |
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| Institution: | 1. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Equipo de Calidad Alimentaria, c/Mayor s/n, La Alberca, 30150 Murcia, Spain;2. Universidad Pública de Navarra, Dpto. de Ciencias del Medio Natural, Campus de Arrosadía, 31006 Pamplona, Spain;1. Institute of Agricultural Engineering, Agricultural Research Organization, The Volcani Center, P.O.B 15159, Rishon LeZion 7528809, Israel;2. Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, The Volcani Center, P.O.B 15159, Rishon LeZion 7528809, Israel;3. Mop Darom, Besor Experimental Station, Mobile Post 4, 85400, Israel;4. HIT – Holon Institute of Technology, POB 305, Holon 58102, Israel;1. IRD, UMR LSTM, 34398 Montpellier, France;2. Laboratoire Commun de Microbiologie IRD/ISRA/UCAD, Dakar, Senegal;1. INFN Sezione di Roma Tor Vergata, Via della Ricerca Scientifica, 1 -00133 Roma, Italy;2. INFN Sezione di Roma, P.le Aldo Moro, 2 -00185 Roma, Italy;1. A. E. Arbuzov Institute of Organic, Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences, 420088 Kazan, Russian Federation;2. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation;1. Department of Chemical and Environmental Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;3. INTROP, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;1. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, IMIDA, C/Mayor s/n, La Alberca, 30150 Murcia, Spain;2. Departamento de Química Agrícola, Geología y Edafología, Facultad de Química, Universidad de Murcia, Campus Universitario de Espinardo, 30100 Murcia, Spain |
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| Abstract: | The contribution of plant growth-promoting bacteria (PGPB) of the genus Azospirillum to the plant N budget through biological nitrogen fixation (BNF) is still controversial. The aim of this study was to determine the contribution of BNF by Azospirillum brasilense on pepper grown at different N levels, attained using the 15N natural abundance method. To this end, pepper plants were grown in a growth chamber and treated with A. brasilense combined with Pantoea dispersa and then irrigated at four different N levels (0, 1, 3 and 7 mM NO3?). The assimilation of fixed N was clear from the lower δ15N values observed in bacteria-treated plants compared with those of non-bacteria treated plants. The percentage of BNF-derived N decreased with decreasing NO3? levels in the growth medium. BNF contribution to the total nitrogen content of plants was found to be as high as 46%. The results suggest that the bacteria have a potential to supply a considerable amount of N to pepper seedlings, as well as to stimulate plant growth and N uptake when Azospirillum–Pantoea treatment is combined with low NO3? levels. |
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