雪莲果叶片愈伤组织的诱导

目的]研究雪莲果叶片愈伤组织诱导的最佳灭菌方法和最佳培养基。方法]以雪莲果叶片为外植体,用浓度0.1%升汞浸泡不同时间,筛选最佳灭菌方法;以MS为基本培养基,比较不同浓度生长调节物质对愈伤组织诱导的影响。结果]用浓度0.1%升汞溶液灭菌8 min,外植体污染率为54.55%,死亡率为3.64%;最佳诱导培养基为MS+6-BA 1.0~1.5 mg/L+NAA 0.5~1.0 mg/L。结论]最佳灭菌方法为浓度0.1%升汞对叶片灭菌8 min;诱导愈伤组织的最佳培养基为MS+6-BA 1.0~1.5 mg/L+NAA 0.5~1.0 mg/L。

Callus Induction of Smallanthus sonchifolius Leaves

Objective] The experiment was carried out to study the optimum sterilizing method and medium in callus induction of Smallanthus sonchifolius leaves.Method] The leaves were sterilized in 0.1% mercuric chloride at different times to seek out the optimum sterilizing method.MS medium with different concentrations of growth regulating substance was adopted to contrast their effects on callus induction.Result] Explants sterilized in 0.1% mercuric chloride for 8 min had the best effect.The optimum medium for callus was MS 6-BA 1.0-1.5 mg/L NAA 0.5-1.0 mg/L.Conclusion] The optimum sterilizing method was sterilizing the leaves for 8min in 0.1% mercuric chloride.And the optimum medium for callus was MS 6-BA 1.01.5 mg/L NAA 0.5-1.0 mg/L.