| 中药天冬叶片DNA的提取方法比较研究 |
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| 引用本文： | 曾桂萍,潘化仁. 中药天冬叶片DNA的提取方法比较研究[J]. 贵州农业科学, 2009, 37(6) |
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| 作者姓名： | 曾桂萍 潘化仁 |
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| 作者单位： | 贵州大学,农学院,贵州,贵阳,550025;贵州省荔波农业局,贵州,荔波,558400 |
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| 基金项目： | 贵州省中药现代化项目 |
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| 摘 要： | 为了能利用ISSR技术在分子水平上研究贵州天冬种质资源的遗传多样性,对天冬叶片DNA的提取方法进行了研究.结果表明:提取液中使用CTAB比SDS所得DNA质量好.研磨时加氯仿可以有效抑制DNA酶的活性和去除蛋白质杂质;用氯仿/异戊醇(24/1)抽提1次可获得质量好、浓度高的DNA,可以满足ISSR分析的需要.
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| 关 键 词： | 天冬 DNA提取 ISSR分析 |
Method of DNA Extraction from Asparagus cochinchinensis Leaf |
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| ZENG Gui-ping,PAN Hua-ren. Method of DNA Extraction from Asparagus cochinchinensis Leaf[J]. Guizhou Agricultural Sciences, 2009, 37(6) |
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| Authors: | ZENG Gui-ping PAN Hua-ren |
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| Affiliation: | 1.Agricultural College;Guizhou University;Guiyang;Guizhou 550025;2.Libo Agricultural Bureau;Libo;Guizhou 558400;China |
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| Abstract: | Four DNA extraction methods from Asparagus cochinchinensis leaves was tested to study the genetic diversity of Asparagus cochinchinensis germplasm resources by using ISSR technique on the molecular level.The results showed that the DNA quality from the extraction solution with 1.5% CTAB is better than that from the extraction solution with 1.5% SDS,adding chloroform can effectively inhibit the activity of DNase and remove the impurity of protein when the sample was grinded,and the DNA with good quality and ... |
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| Keywords: | Asparagus cochinchinensis DNA extract ISSR analysis |
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