盐胁迫对刺槐幼苗叶绿素荧光及叶片解剖结构的影响

为了研究盐胁迫对刺槐幼苗光合作用以及细胞形态结构的影响,探索刺槐耐盐胁迫的生理机制和不同品种之间的差别,为干旱盐碱地区生态树种推广提供技术支撑。本研究采用盆栽实验,以“豫引1号”和“3-I”两个刺槐品种的幼苗为试验材料,对其进行0nmol/L,200nmol/L,400nmol/L三个浓度梯度的NaCl胁迫处理,利用dualPAM-100叶绿素荧光仪研究NaCl胁迫对刺槐幼苗叶绿素荧光特性的影响,同时通过显微电镜观察研究NaCl胁迫对刺槐幼苗叶片解剖结构的影响。结果表明在受到不同浓度NaCl胁迫后,“豫引1号”和“3-I”的差异十分显著,两个品种的F_m值和叶片解剖结构在不同浓度胁迫之间存在显著差异。综合分析,“3-I”受NaCl胁迫影响较大,受害较重,而“豫引1号”具有一定的抗盐能力,在400nmol/L NaCl胁迫下表现出较强的适应性。

Effects of Salinity on Chlorophyll Fluorescence Characteristics and Leaf Anatomical Structure of Robinia pseudoacacia L. Cutting Seedlings

To study the effects of salt stress on photosynthetic characteristics and cell structure of Robinia pseudoacacia L. cutting seedlings and explore the salt resistance photosynthetic characteristics of differentR. pseudoacacia varieties and the difference among varieties, twoR.pseudoacacia varieties’seedlings were taken as test materials, after NaCl treatment, the effects of NaCl stress on chlorophyll fluorescence characteristicsand leaf anatomical structure were studied. The results showed that there were significant differences of Fm value under different NaCl concentrations. ΦPS Ⅱ , ETR and qp gradually decreased with the NaCl concentration increase.NPQ sharply rose and showed a significant difference. With the NaCl concentration increase, CTR of‘Yuyin No.1’significantly increased and SR decreased compared with control, and the stomata opened. But the leaves of‘3-I’were wilted and deactivated, and the stomata closed. In general,‘3-I’ is greatly influenced by NaCl stress, while‘Yuyin No.1’has salt resistance and shows a strong adaptability under NaCl stress.