甲基营养型芽胞杆菌NKG-1对番茄白粉病的防病促生作用研究

研究旨在探讨甲基营养型芽胞杆菌NKG-1对番茄白粉病防病和促生的双重作用。试验采用灌根法研究了不同菌体密度的NKG-1对番茄的促生效果,采用喷雾法研究了NKG-1对番茄白粉病的预防和治疗效果。温室盆栽试验结果表明,NKG-1菌体密度在1×10~7cfu/mL~1×10~9cfu/mL间对植株促生效果最明显,根长最大较对照提高55.01%,鲜重最大较对照提高35.62%。保护地番茄促生试验表明,植株坐果数较对照提高28.57%,冠幅周径提高16.72%。保护地番茄白粉病防治试验表明,在白粉病发生前预防性喷施NKG-1对番茄白粉病防效达87.55%,白粉病发生后喷施NKG-1治疗效果为47.74%。综上所述,NKG-1对番茄有良好的防病促生作用,且对白粉病的预防效果显著优于治疗效果,该研究为菌株的大面积推广应用提供了理论基础。

Bacillus methylotrophicus strain NKG-1: Growth Promotion and Disease Control Effect on Tomato Powdery Mildew

The paper aims to explore the growth promotion and disease control effects of Bacillus subtilis strain NKG-1 on tomato powdery mildew. The effects of NKG-1 fermentation broth with different microbial density on growth promoting by irrigation were studied. And the preventive and treatment effects of NKG-1 on tomato powdery mildew by spray were analyzed. Potted tomatoe test showed that: the most effective microbial density was between 1×107cfu/mL to 1×109cfu/mL, under which the seedling fresh weight increased by 35.62% and the root length increased by 55.01% compared with the control. The greenhouse growth promotion test showed that: the number of fruit set was 28.57% higher than that of the control, and the crown width increased by 16.72%. Furthermore, the greenhouse disease control test showed that: the preventive effect of sprayed NKG- 1 fermentation broth to tomatoes before powdery mildew onset was 87.55%, the treatment effect on tomato after powdery mildew onset was 47.74%. These results indicate that NKG- 1 has a significant effect on growth promotion and disease control of tomatoes, and the preventive effect of powdery mildew is better than that of treatment. This study provides a theoretical basis for the large-scale application of NKG-1 to tomatoes.