二月兰花瓣花色苷组成分析

为了研究二月兰花色苷成分组成特点，本试验以3份二月兰为试材，利用特征颜色反应确定二月兰花瓣的色素类型，并通过高效液相色谱-质谱联用技术(HPLC-Q-TOF-MS)分析花色苷种类及其含量。研究结果表明，3份二月兰花瓣中均不含类胡萝卜素，除白色花瓣以外，其余均含花色苷。蓝紫色花瓣的花色苷含量最高，粉色次之。在二月兰花瓣中共检测到了7种花色苷成分，通过与已有文献比对，推定其成分为矢车菊素3-芸香糖苷、锦葵素3,5-二葡萄糖苷、矢车菊素3-香豆酰葡萄糖苷-5-葡萄糖苷、矢车菊素3-p-香豆酰二葡萄糖苷-5-葡萄糖苷、矢车菊素3-阿魏酰槐糖苷-5-丙二酰葡萄糖苷、矢车菊素3-香豆酰槐糖苷-5-丙二酰葡萄糖苷、矢车菊素3-(6′-丙二酰葡糖苷-2′-(6′′-香豆酰-2′′-羟基苯甲酰葡糖苷))-5-丙二酰葡糖苷。粉色花瓣中仅含矢车菊素的花色苷衍生物，而蓝紫色花瓣中则含矢车菊素和锦葵素的花色苷衍生物。本研究为二月兰花色苷成分的进一步分离和鉴定工作提供了参考，同时也为二月兰花色的分子育种提供理论依据。

Anthocyanin Profiles in Petals of Orychophragonus violaceus

The paper aims to study anthocyanin component characteristics in Orychophragonus violaceus. Petal pigment type was identified by characteristic color reaction, and the UPLC-Q-TOF-MS technology was used for qualitative and quantitative analysis of anthocyanins in threeO. violaceus materials. The results suggested that colored carotenoids were not detected in petals of all cultivars, and blue purple and pink petals contained anthocyanins in petals. Blue purple petals contained the highest anthocyanin content, followed by pink petals. A total of seven anthocyanin components were detected in petals of three cultivars, including cyanidin 3- rutinosid, malvidin 3,5- glucoside, cyanidin 3- coumaroylglucoside- 5- glucoside, cyanidin- 3- p - coumaroyldiglucoside- 5- glucoside, cyanidin 3- feruloylglucoside- 5- malonylglucoside, cyanidin 3-coumarylsophoroside- 5- malonylglucoside, and cyanidin 3- (6′- malonylglucoside- 2′- (6′′- coumaryl- 2′′- hydroxybenzoylglucoside))-5-malonylglucoside. Pink petals contained only cyanidin derivated anthocyanins while blue purple petals contained majorly cyanidin derivated anthocyanins and some malvidin derivated anthocyanins. This paper provides a reference for the identification of molecular structure of anthocyanins and the molecular breeding of flower color in O. violaceus.