| 抗鹅IgY抗血清制备及在ELISA中的应用 |
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| 引用本文： | 罗嘉琪,田允波,江丹莉,刘文俊,黄运茂.抗鹅IgY抗血清制备及在ELISA中的应用[J].仲恺农业工程学院学报,2018,31(2):8-12. |
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| 作者姓名： | 罗嘉琪 田允波 江丹莉 刘文俊 黄运茂 |
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| 作者单位： | 广东省水禽健康养殖重点实验室;仲恺农业工程学院动物科技学院 |
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| 摘 要： | 为制备鹅免疫球蛋白特异性抗体，通过主动免疫兔子制备了抗鹅ＩｇＹ抗血清，并在ＥＬＩＳＡ中比较分析了该抗血清的应用效果．结果显示，将制备的抗鹅ＩｇＹ抗血清作为二抗，当抗原包被浓度（１６μｇ／ｍＬ）和血清（１∶１６００）、抗鹅ＩｇＹ抗血清（１∶５１２００）与酶标抗体（１∶２０００）稀释到合适浓度时，在ＥＬＩＳＡ检测鹅抗体水平中呈现出良好效果，其特异性和灵敏度明显好于以兔抗鸡ＩｇＹ作为替代二抗．结果表明所制备的抗鹅ＩｇＹ抗血清在鹅相关的酶联免疫研究方法中是合格和有效的．
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Preparation of anti-goose IgY antiserum and its application in ELISA |
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| Abstract: | In order to prepare goose specific antibodies for goose research，anti-goose IgY antiserum wasprepared by active immune rabbit，and the effect of the antiserum was compared and analyzed in ELISA．The results showed when the anti-goose IgY antiserum was prepared as the second antibody，the coatingantigen( 16 μg/mL) ，serum( 1∶ 1 600) ，anti-goose IgY antiserum( 1∶ 51 200) and enzyme labelled anti-body( 1∶ 2 000) diluted to appropriate concentration，it showed a good effect in ELISA for detecting gooseantibody levels，and the specificity and sensitivity were better than that with Rabbit anti chicken IgY asan alternative two resistance． It showed that the anti-goose anti IgY antiserum prepared by this study wasqualified and effective in the geese related enzyme linked immunosorbent assay． |
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