番茄枯萎病菌基因组微卫星位点分析与引物设计

通过对前期测定的番茄枯萎病菌Ｆｕｓａｒｉｕｍｏｘｙｓｐｏｒｕｍｆ．ｓｐ．ｌｙｃｏｐｅｒｓｉｃ基因组１４５０１条ｇｅｎｅ序列进行搜索，发现４６６个简单重复序列（Ｓｉｍｐｌｅｓｅｑｕｅｎｃｅｒｅｐｅａｔ，ＳＳＲ）分布在４２５条ｕｎｉｇｅｎｅｓ中，发生频率为２.９３％．在番茄枯萎病基因组的ＳＳＲ中，三核苷酸重复占主导地位，占ＳＳＲ总数的６５.８８％．优势重复单元为ＡＧＣ／ＣＴＧ和ＡＡＧ／ＣＴＴ，分别占１６.５２％和１２.６６％．基于筛选的ＳＳＲｓ，运用Ｐｒｉｍｅｒ３软件进行引物的批量设计，共设计出４５１对引物．结果说明番茄枯萎病菌基因组中含有丰富的ＳＳＲ位点．

Analysis of SSR genetic markers and primer designed from the genome of Fusarium oxysporum f. sp. lycopersic

A total of 14 501 unigenes in Fusarium oxysporum f. sp. lycopersic were screened by using bioinformatics software to search for Simple sequence repeat (SSR) motifs, 466 SSRs were sought out, distributing in 425 unigenes and the occurrence freguency was 2. 93%. Trinucleotide repeats were major types among the obtained unigenes, accounting for 65. 88%. AGC/CTG (16. 52%) and AAG/CTT (12. 66%) were the most abundant motifs. Based on the flanking sequences, 451 primer pairs were designed by using Premier 3 software. These results showed that it is an effective and feasible approach to developing SSR markers based on genome database in F. oxysporum f. sp. lycopersic, and that there were abundant SSR loci in the genome of F. oxysporum f. sp. lycopersic.