| 密花石斛无菌播种与茎尖培养研究 |
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| 引用本文： | 邓选国,黄俊鹏,宋希强,凌绪柏. 密花石斛无菌播种与茎尖培养研究[J]. 热带作物学报, 2009, 33(5): 41-43 |
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| 作者姓名： | 邓选国 黄俊鹏 宋希强 凌绪柏 |
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| 作者单位： | 1 海南大学园艺园林学院；2 中国热带农业科学院后勤服务中心;三亚市安游4802工厂;1 海南大学园艺园林学院；3 海南省热带花卉研究所;海南省热带花卉研究所 |
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| 摘 要： | 密花石斛是原产海南、兼具观赏和药用价值的野生石斛。为促进其规模化生产，探讨了密花石斛的无菌播种和茎尖培养中培养基种类和激素等配比。结果表明：在花宝1号附加土豆的培养基上萌芽效果最好，原球茎发生率较高；分化阶段以花宝1号附加香蕉的培养基分化效果最理想，幼嫩茎尖在MS基本培养基上附加BA 3.0 mg/L与NAA 0.2 mg/L上可诱导出新芽。
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| 关 键 词： | 密花石斛 ；无菌播种 ；茎尖培养 |
Asymbiotic Germination and Shoot Tip Culture of Dendrobium densiflorum Lindl |
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| DENG Xuanguo,HUANG Junpeng,SONG Xiqiang and LING Xubo. Asymbiotic Germination and Shoot Tip Culture of Dendrobium densiflorum Lindl[J]. Chinese Journal of Tropical Crops, 2009, 33(5): 41-43 |
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| Authors: | DENG Xuanguo HUANG Junpeng SONG Xiqiang LING Xubo |
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| Abstract: | Dendrobium densiflorum Lindl. is indigenous to Hainan Island and has both ornamental and medical value. Cultural media and hormones were selected for asymbiotic germination and shoot tip culture in vitro of D. densiflorum for commercial propagation. The asymbiotic germination indicated that the medium Hyponex No.1 was optimum for PLB formation when supplemented with potato juice, and optimum for PLB differentiation when supplemented with banana juice. Fresh sprouts were induced from the explant of the shoot tip cultured on the MS medium in the presence of BA 3.0 mg/L and NAA 0.2 mg/L. |
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