| 2种荔枝RNA提取方法的比较 |
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| 引用本文： | 印 华,周兆德,吴志祥. 2种荔枝RNA提取方法的比较[J]. 热带作物学报, 2009, 33(2): 1-3 |
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| 作者姓名： | 印 华 周兆德 吴志祥 |
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| 作者单位： | 1 中国热带农业科学院橡胶研究所；2 海南大学环植学院;海南大学环植学院;中国热带农业科学院橡胶研究所 |
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| 基金项目： | 国家自然科学基金（No.30560065）、海南省自然科学基金（No.30402）和海南省教育厅高校教师基金（No.Hjkj2009-27）资助 |
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| 摘 要： | 比较利用TRIZOL法和改进SDS法2种方法提取荔枝叶片总RNA的可行性。通过琼脂糖凝胶电泳及紫外分光光度计检测，对提取所得RNA的完整性、纯度及浓度进行分析。试验结果表明，改进SDS法适合荔枝叶片总RNA的提取，能有效获得纯度高、完整性好的RNA样品。
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| 关 键 词： | 荔枝；TRIZOL法；改进SDS法；总RNA ；提取；纯度 |
Comparison of Two Methods for Extracting RNA from Litchi |
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| YIN Hu,ZHOU Zhaode and WU Zhixiang. Comparison of Two Methods for Extracting RNA from Litchi[J]. Chinese Journal of Tropical Crops, 2009, 33(2): 1-3 |
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| Authors: | YIN Hu ZHOU Zhaode WU Zhixiang |
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| Abstract: | Two methods, TRIZOL extraction and modified SDS, were applied to isolate total RNA fromthe leaf of Litchi. The total RNA isolated by these two methods was detected by the agarose gel electrophoresis and the ultraviolet spectrophotometry for analysis of its integrity, purity and concentration. The results showed that the modified SDS method is more effective and can produce leaf total RNA with integrality and high purity. |
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| Keywords: | Litchi TRIZOL extraction method modified SDS method total RNA extraction purity |
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