促生细菌挥发性有机物调控根部质膜H+-ATPase 活性提高植物耐碱胁迫能力

根际促生细菌的挥发性有机化合物（VOCs）可以促进植物生长并提高植物对盐、旱和病菌的抵抗力。分析了芽孢杆菌PDR1产生的VOCs对拟南芥耐碱胁迫能力的影响及其生理机制。在芽孢杆菌PDR1存在或不存在的条件下，对拟南芥进行培养；检测对照和碱胁迫条件下拟南芥根部的各种离子含量；用pH指示剂（溴甲酚紫）和pH计检测拟南芥的根际酸化能力；检测拟南芥根部质膜H⁺-ATPase活性。结果显示，芽孢杆菌PDR1的VOCs改善了同一空间中碱胁迫下拟南芥的根长和根部离子平衡；来自芽孢杆菌PDR1的VOCs增强了拟南芥根中质膜H⁺-ATPase的活性，并提高了拟南芥植物的根际酸化能力。研究结果将促进对芽孢杆菌调节拟南芥生长和抗逆性机制的理解，并为其在农业生产和生态保护中的广泛应用奠定基础。

Volatile organic compounds from growth-promoting bacteria promote arabidopsis root plasma membrane H+-ATPase activity and improve plant alkali tolerance

Volatile organic compounds (VOCs) from rhizosphere-promoting bacteria can promote plant growth and increase plant resistance to salt, drought and pathogens. The effects of VOCs produced by Bacillus .spp PDR1 on arabidopsis plasma membrane H⁺-ATPase activity and its physiological mechanisms were analyzed by culturing Arabidopsis thaliana plants in the presence or absence of PDR1. The rhizosphere acidification ability of Arabidopsis thaliana was detected by pH indicator (bromocresol purple) or pH meter; the ion content in arabidopsis roots under the control and alkali stress conditions was also detected, and the plasma membrane H⁺-ATPase activity of arabidopsis root was measured. The results showed that the VOCs of PDR1 enhanced the resistance of arabidopsis to alkaline stress. VOCs from PDR1 enhanced the activity of plasma membrane H⁺-ATPase in arabidopsis roots and increased the ability of rhizosphere acidification of arabidopsis plants. Our findings can promote understanding of the mechanisms by which Bacillus. spp regulates the growth and stress tolerance of arabidopsis, and lay a foundation for its widespread use in agricultural production and ecological conservation.