响应面法优化酶法提取大鲵皮胶原蛋白工艺

为了优化人工养殖大鲵(Andrias davidianus Blanchard)皮中胶原蛋白的提取工艺, 以加酶量、液料比和酶解时间为试验因素, 胶原蛋白提取率为响应值, 采用Box-Behnken设计进行试验。通过响应面法考察了3个因素对胶原蛋白提取率的影响, 建立了胶原蛋白提取率的二次多项式回归模型, 并对提取工艺进行了优化。试验结果表明, 3个因素对鲵皮中胶原蛋白提取率的影响大小次序为: 液料比>酶解时间>加酶量; 交互项中“液料比-酶解时间”项对提取率影响显著, 其他交互项对提取率影响不显著。为满足实际需要, 对模型预测得到的大鲵皮胶原蛋白最优提取工艺稍做调整, 具体参数为: 加酶量16.5%、液料比15 mL/g、酶解时间29 h。此条件下, 大鲵皮胶原蛋白提取率可达到66.99%, 与模型理论预测值(67.83%)较为一致。

Optimization of conditions for the extraction of pepsin-soluble collagen from the skin of Chinese giant salamander (Andrias davidianus)

We evaluated the optimal conditions for extraction of pepsin-soluble collagen from the skin of Chinese giant salamander (Andrias davidianus) using a Box-behnken design experiment. The concentration of pepsin, solvent-to-solid ratio, and enzyme extraction time were the test factors and the extraction rate of collagen was the target parameter. We used response surface methodology to describe the influence of the three factors on the extraction rate of collagen and developed aquadratic polynomial regression model to optimize the extraction conditions. The order of influence of the factors was: solvent-to-solid ratio>enzyme extraction time>pepsin amount. The interaction between the solvent-to-solid ratio and enzyme extraction time had a significant effect on the extraction rate of collagen, while other interactions had no significant effect. The optimal conditions predicted by the regression model for extraction were slightly adjusted to meet actual production. The specific parameters were: pepsin concentration was 16.5%, solvent-to-solid ratio was 15 mL/g, and enzyme extraction time was 29 h. Under these conditions the extraction rate was 66.99%, which was similar to the theoretical value (67.83%) predicted by the regression model.