大孔树脂纯化柠条花中总生物碱的工艺研究

以柠条花为原料，采用大孔树脂分离纯化柠条花中总生物碱。通过对比6种不同型号大孔树脂对总生物碱吸附-解吸效果及静态动力学研究，确定AB-8大孔树脂为柠条花中总生物碱最佳纯化材料。通过单因素试验确定其对柠条花中总生物碱动态吸附-解吸最佳工艺条件为：上样液浓度为 2 mg/mL，上样pH为6.0，上样流速为2 BV/h；解吸剂为90%乙醇，解吸流速1.5 BV/h，解吸剂用量3 BV。在此条件下，柠条花中总生物碱分离纯化效果最佳，纯度为12.57%，表明AB-8大孔树脂对柠条花总生物碱具有较好的纯化效果。

Purification of Total Alkaloids from Flower of Intermediate Peashrub by Macroporous Resin

Total alkaloids was isolated and purified by macroporous resin from flower of intermediate peashrub. Six types of different macroporous resins were compared for the adsorption-desorption effect and static kinetics of total alkaloids to determine the AB-8 macroporous resin as the best purification materials for total alkaloids from lower of intermediate peashrub. The optimal parameters for dynamic adsorption-desorption of total alkaloids determined by single factor experiments were as follows: sample concentration 2 mg/mL, sample pH 6.0, and sample flow rate 2 BV/h; desorption agent 90% ethanol, desorption flow rate 1.5 BV/h, desorption agent dosage 3 BV. Under these conditions, the purification effect of total alkaloids from the flowers was the best with a purity of 12.57%. Which indicated that AB-8 macroporous resin had a better effect in purifying total alkaloids from flower of intermediate peashrub.