水稻新型卷叶突变体rl12（t）的遗传分析和基因定位

叶片是水稻光合作用的重要器官，适度卷曲有利于改善群体光照、提高光能利用率，卷叶基因是培育理想株型的重要资源。本研究利用EMS诱变优良恢复系缙恢10号，获得了一个水稻新型卷叶突变体，该性状受一对显性基因控制，表现为新叶不卷，老叶全卷，而成熟叶片叶上部约1/3卷曲、中下部正常，叶绿素含量极显著高于对照，暂被命名为rl12(t)。利用SSR标记将该基因定位于第10染色体SWU-1和SWU-2之间，遗传距离分别是1.5 cM和0.2 cM。目前，类似于rl12(t)卷叶突变体表型未见报道，RL12(t)是唯一一个在第10染色体被分子定位的显性卷叶主基因。研究结果为该卷叶基因的克隆和功能分析奠定了基础，对于揭示卷叶机理及应用于株型改良具有重要的意义。

Genetic Analysis and Gene Mapping of a Novel Rolled Leaf Mutant rl12(t) in Rice

Leaf is an important organ in photosynthesis, its moderate rolling could facilitate the improvement of plant population's structure and enhance light-use efficiency, which is significant in ideotype breeding. Recently, more attention has been paid to the identification of rice rolled leaf mutants and related gene cloning by breeders and geneticists. This paper reported a rolled leaf mutant, temporarily named rl12(t), from the restorer line Jinhui10 treated by EMS. In the mutant, the initializing leaves did not roll, the mature leaves curled the upper 1/3 section of them and the older mature leaves rolled completely, the pigment contents increased significantly. To date, such phenotype has never been reported. One CMS line Xinong 1A with normal leaves was crossed with the rl12(t) mutant, the F1/F2 populations were used for genetic analysis, suggesting that the mutant trait were controlled completely by one single dominant nuclear gene. rl12(t) was finally located on the chromosome 10 between SWU-1 and SWU-2 with genetic distances of 1.5 and 0.2 cM, respectively. There has been no rolled leaf gene reported on this chromosome. Therefore, the RL12(t) should be novel, and also the unique dominant rolled leaf gene mapped by molecular markers up to now. The results provide a basis for RL12(t) gene cloning and functional analysis, as well as mechanism studies of rolled leaf and the application in plant-type breeding.