| 花斑皮蠹和黑斑皮蠹(鞘翅目:皮蠹科)的快速分子鉴定 |
| |
| 引用本文： | 刘聪,蒋湘,樊武疆,王新国,吕英姿,林惠娇,刘二龙,夏来群,班丽萍.花斑皮蠹和黑斑皮蠹(鞘翅目:皮蠹科)的快速分子鉴定[J].北京农学院学报,2012,27(2):27-30. |
| |
| 作者姓名： | 刘聪 蒋湘 樊武疆 王新国 吕英姿 林惠娇 刘二龙 夏来群 班丽萍 |
| |
| 作者单位： | 中国农业大学农学与生物技术学院,北京,100193;中华人民共和国黄埔出入境检验检疫局,广州,510730;中国农业大学动物科技学院,北京,100193 |
| |
| 基金项目： | 广东检验检疫局2010年科技项目，中央高校基本科研业务费专项资金 |
| |
| 摘 要： | 斑皮蠹属昆虫个体小,形态高度相似,仅依据外部形态难以鉴定。用线粒体细胞色素氧化酶亚基I(COI)基因的通用引物,分别以黑斑皮蠹(Trogoderma glabrum)、花斑皮蠹(Trogoderma variabile)的DNA为模板,进行PCR扩增、测序。比较二者COI的碱基序列,找出所有序列多态性位点及相应的限制性内切酶,最终选取限制性内切酶AflⅡ对二者进行酶切。结果表明,黑斑皮蠹的COI序列可被限制酶AflⅡ切开,形成535bp和347bp两部分,而花斑皮蠹COI片段中因不具有AflⅡ酶切位点而不能被切开,从而将二者成功区分,实现对黑斑皮蠹和花斑皮蠹的快速分子鉴定。
|
| 关 键 词： | 黑斑皮蠹 花斑皮蠹 COI基因 酶切 分子鉴定 |
Rapid Molecular Identification of Trogoderma glabrum and Trogoderma varia bile ( Coleoptera : Dermestidae) |
| |
| LIU Cong
,
JIANG Xiang
,
FAN Wu-jiang
,
WANG Xin-guo
,
LU Ying-zi
,
LIN Hui-jiao
,
LIU Er-long
,
XIA Lai-qun
,
BAN Li-ping.Rapid Molecular Identification of Trogoderma glabrum and Trogoderma varia bile ( Coleoptera : Dermestidae)[J].Journal of Beijing Agricultural College,2012,27(2):27-30. |
| |
| Authors: | LIU Cong JIANG Xiang FAN Wu-jiang WANG Xin-guo LU Ying-zi LIN Hui-jiao LIU Er-long XIA Lai-qun BAN Li-ping |
| |
| Institution: | 1.College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China;2.Huangpu Entry-Exit Inspection and Quarantine Bureau,Guangzhou,510730,China;3.College of Animal Science&Technology,China Agricultural University,Beijing 100193,China) |
| |
| Abstract: | In this paper,DNA isolated from species of T.glabrum and T.variabile was amplified by polymerase chain reaction(PCR),the products were directly sequenced,analyzed,and digested with restriction enzyme AflⅡ.The results proved that COI sequences of T.glabrum could be digested into two parts: 535bp and 347 bp,but T.variabile was not.Thus,T.glabrum and T.variabile were identified accurately and efficiently. |
| |
| Keywords: | Trogoderma glabrum Trogoderma variabile mitochondrial cytochrome oxidase gene subunit I restriction enzyme digestion molecular identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
