Successful Hybridization of Acipenser Species Using Cryopreserved Sperm |
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Authors: | Béla Urbányi Ákos Horváth Balázs Kovács |
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Institution: | (1) Department of Fish Culture, Faculty of Agriculture and Environmental Sciences, Szent Istvan University, Páter K. u. 1., Gödöllö, H-2103, Hungary;(2) Szent-Györgyi Albert ut 4, Agricultural Biotechnology Center, Gödöllö, H-2100, Hungary |
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Abstract: | Successful hybridization of sterlet (Acipenser ruthenus) × Siberian sturgeon (Acipenser baeri), sterlet × Russian sturgeon (Acipenser gueldenstaedti) and sterlet × European sturgeon (Acipenser sturio) was carried out for the first time using cryopreserved semen of sturgeon males and sterlet × sterlet crosses as controls. Sperm of all three species was diluted with a cryodiluent composed of 23.4 mM sucrose, 0.25 mM KCl, 30 mM Tris (pH 8.0) and 10% methanol. The samples were frozen in plastic straws in the vapor of liquid nitrogen at the height of 3 cm above the level of nitrogen for 3 min. Following thawing approximately 3000 sterlet eggs were fertilized with six straws of frozen-thawed sperm. The hatching rate with sterlet sperm was 30.6% while the hatching rate of A. ruthenus × A. baeri, A. ruthenus × A. gueldenstaedti and A. ruthenus × A. sturio hybrids was 50, 17.4 and 34%, respectively. Morphometric markers as well as random amplified polymorphic DNA (RAPD) assay was used to verify interspecific hybridization. |
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Keywords: | Cryopreservation Hybrids RAPD Sperm Sturgeon |
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