| 辽宁省猪源弓形虫虫株分离与鉴定 |
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| 引用本文： | 杨娜,邢蒙恩,王大为,吴元华. 辽宁省猪源弓形虫虫株分离与鉴定[J]. 华中农业大学学报, 2017, 36(2): 84-88 |
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| 作者姓名： | 杨娜 邢蒙恩 王大为 吴元华 |
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| 作者单位： | 1. 沈阳农业大学植物保护学院,沈阳110866;沈阳农业大学畜牧兽医学院,沈阳110866;2. 沈阳农业大学畜牧兽医学院,沈阳,110866;3. 沈阳农业大学植物保护学院,沈阳,110866 |
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| 基金项目： | 国家自然科学基金青年基金项目（31201894）; 中国博士后科学基金面上项目（2014M561252） |
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| 摘 要： | 以采自辽宁省某屠宰场2 063头猪的血清和心脏组织为试验材料,采用改良凝集试验(MAT)对血清进行定量检测和弓形虫虫株分离,结果显示:2 063份猪血清中阳性血清(滴度≥25)共计233份,阳性检出率为11.3%;选取阳性抗体滴度≥100所对应的67份猪心脏组织进行弓形虫虫株分离,结果从猪心脏组织中共分离出23株弓形虫虫体,分离率为34.3%。此结果提示,辽宁地区猪弓形虫感染率较高,对该地区人们的健康存在潜在威胁。
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| 关 键 词： | 弓形虫 猪 虫株分离 MAT PCR |
| 收稿时间： | 2016-06-15 |
Isolation and identification of Toxoplasma gondii strains from pigs in Liaoning Province |
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| YANG Na,XING Meng,#;en,WANG Dawei,WU Yuanhua. Isolation and identification of Toxoplasma gondii strains from pigs in Liaoning Province[J]. Journal of Huazhong Agricultural University, 2017, 36(2): 84-88 |
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| Authors: | YANG Na,XING Meng&# en,WANG Dawei,WU Yuanhua |
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| Affiliation: | YANG Na,XING Meng'en,WANG Dawei,WU Yuanhua |
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| Abstract: | Serum from 2 063 pigs from a slaughterhouse in Liaoning Province were collected and detected by modified agglutination test (MAT).The results showed that the samples with titer≥25 were 233 and the positive rate was 11.3 ％.A total of 67 pigs with titer≥100 were chosen for Toxoplasma gondii isolation,in which 23 T.gondii strains were isolated from the heart tissues with an isolation rate of 34.3％.In this study,T.gondii strains were isolated successfully,showing the infectious status of pig toxoplasmosis in Liaoning Province,which poses a huge potential threat to public health. |
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| Keywords: | Toxoplasma gondii pigs isolation modified agglutination test (MAT) polymerase chain reaction(PCR) |
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