番茄灰霉病菌胁迫下壮观链霉菌SC11对寄主的促生作用及防病机理

 放线菌SC11是一株从土壤中分离的具有广谱抑菌活性的壮观链霉菌。平板对峙试验表明SC11能显著抑制离体灰葡萄孢的生长;盆栽试验表明,SC11对番茄灰霉病的防病效果达到65.3%,高于对照药剂腐霉利(55.8%);对番茄植株内防御酶活性、丙二醛(MDA)和脯氨酸含量的测定表明,SC11处理可诱导植株叶片组织防御酶活性、脯氨酸含量等升高,而MDA含量降低;SC11还能明显促进番茄幼苗的生长,株高、茎粗、叶绿素含量,和对照相比均达显著水平。上述结果表明,壮观链霉菌SC11能有效防治番茄灰霉病,诱导植株防御酶活性的提高,促进番茄幼苗生长。

The disease prevention and growth-promoting effect and mechanism of Streptomyces spectabilis SC11 to Botrytis cinerea stress

Streptomyces spectabilis SC11, isolated from soil, has a broad spectrum of antibacterial activity. The control efficacy of SC11 against tomato gray mold was examined by pair culturing on PDA and pot experiments. Pair culturing experiment on PDA showed that SC11 significantly inhibited the growth of Botrytis cinerea in vitro. Pot experiments showed that the control efficacy of SC11 was 65.3%, higher than that of procymidone (55.8%) treatment. The experiments on defense enzymes, MDA and proline in tomato plant showed that the activity of defense enzymes and the content of proline in tomato leaf were induced, but the content of MDA was decreased. Moreover, SC11 obviously promoted the growth of tomato seedlings, plant height, stem diameter and chlorophyll content. These results indicated that SC11 could not only effectively control tomato gray mold, but also improve the defense enzyme activities in plant and promote the growth of tomato seedlings.