宁夏马铃薯黄萎病病原菌分离鉴定及寄主范围测定

 为明确引起宁夏马铃薯黄萎病病原菌种类,本试验通过常规组织分离法对采自宁夏马铃薯主栽地区固原和西吉的27株马铃薯黄萎病标样进行了分离,共获得11株分离物。采用形态学并结合肌动蛋白(ACT)、延伸因子-1α(EF1α)、3-磷酸甘油醛脱氢酶(GPD)、色氨酸合成酶(TS)的多基因系统发育分析方法,11株分离物均被鉴定为非苜蓿轮枝菌(Verticillium nonalfalfae)。致病性测定结果表明,该轮枝菌能够侵染马铃薯植株幼苗并引致典型的黄萎病症状。非苜蓿轮枝菌对茄科、菊科、豆科、十字花科、葫芦科和锦葵科等6科13种双子叶作物的致病性测定结果表明,其对西葫芦、黄瓜、茄子、烟草、豇豆、棉花和向日葵的致病性强,对番茄、辣椒、生菜和白菜致病力弱且发病缓慢,对西蓝花和苜蓿无致病性。

Isolation and identification of potato Verticillium wilt pathogen in Ningxia and its host range

In order to determine the Verticillium species caused potato Verticillium wilt in Ningxia, China, 27 samples with symptom of Verticillium wilt were collected from Guyuan and Xiji in Ningxia. A total of 11 Verticillium spp. strains were recovered from the samples by tissue isolation method. All isolates were identified as Verticillium nonalfalfae based on morphological characteristics and based on multigene phylogenic analysis of the partial sequences of actin (ACT), elongation factor 1-alpha (EF1α), glyceraldehyde-3-phosphate dehydrogenase (GPD) and tryptophan synthase (TS) genes. Pathogenicity test indicated that V. nonalfalfae could infect potato seedlings and caused typical symptoms of Verticillium wilt. Pathogenicity test of V. nonalfalfae to 13 dicotyledonous crops of 6 families including Solanaceae, Asteraceae, Leguminosae, Cruciferae, Cucurbitaceae, Malvaceae showed that V. nonalfalfae exhibiting high pathogenicity to summer squash, cucumber, eggplant, tobacco, cowpea, cotton and sunflower, and moderate pathogenicity to tomato, pepper, lettuce, and cabbage. However, V. nonalfalfae has no pathogenicity to broccoli and lucerne.