芦蒿细菌性软腐病的病原鉴定

芦蒿是一种因其具有独特气味而广受市民欢迎的野菜,尤其在南京被广泛种植与消费。南京的八卦洲地区每年因芦蒿可获得数亿的产值。自2012年,该地区芦蒿在8~9月份相继出现一种新病害。病害发生初期,扦插在土壤下部的植株茎秆外皮变褐色,伴有臭味,随着病害发生严重,植株表现枯萎直至全株死亡。在八卦洲每年该病害的发生率在10%~20%左右,部分田块绝产。本研究采集发病植株,进行组织分离,通过科赫氏法则验证,共获得了30余株具有致病性的分离物。随机挑选了12株细菌,通过生理生化反应、Biolog测定以及多位点序列分析,将该病原菌鉴定为胡萝卜软腐果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.carotovorum)。本研究为芦蒿细菌性软腐病的首次报道。

Identification of the pathogen causing soft rot of seleng worm wood

Seleng wormwood (Artemisia selengensis Turcz. ex Bess.) is a fragrant edible plant that is widely cultivated in China, especially in Nanjing. In the Baguazhou area of Nanjing, the seleng wormwood industry is estimated to be worth hundreds of millions RMB annually. Since 2012, a bacterial soft rot has been observed annually on seleng wormwood (cv. Dayebai) during the months of August and September. Black, necrotic areas with a foul odor appeared on the stems in soil and resulted in wilt and death of the plants. Disease incidences ranged from 10% to 20% in the affected fields and some seriously damaged fields could be no yield. From 2012, more than 30 bacterial strains with consistent appearance were isolated from symptomatic seleng wormwood samples and 12 of them from different fields were selected for characterization. Based on morphological observation,physiological,biochemical and Biolog test, as well as multilocus sequence analysis, the causal pathogen was identified as Pectobacterium carotovorum subsp. carotovorum.