|
|||||
|
|
| 梨泡状溃疡类病毒的RT-PCR检测及序列分析 | |
| 引用本文： | 朱慧,王国平,杨作坤,文立红,陈婕,徐文兴,洪霓. 梨泡状溃疡类病毒的RT-PCR检测及序列分析[J]. 植物病理学报, 2017, 47(4): 558-562 |
| 作者姓名： | 朱慧 王国平 杨作坤 文立红 陈婕 徐文兴 洪霓 |
| 作者单位： | 华中农业大学植物科技学院,湖北省作物病害监测与安全控制重点实验室,武汉 430070; 华中农业大学,农业微生物学国家重点实验室,武汉430070 |
| 基金项目： | 国家农业行业专项计划项目(nyhyzx201203076-03)和农业产业技术体系(CARS-29-10) |
| 摘 要： | 正梨泡状溃疡类病毒(Pear blister canker viroid,PBCVd)为马铃薯纺锤块茎类病毒科(Pospiviroidae)苹果锈果类病毒属(Apscaviroid)成员~([1])。该类病毒在自然条件下主要侵染野生和栽培的梨和苹果~([2~4]),梨和榅桲多数品种感染PBCVd后无明显症状,通过嫁接传染该类病毒可诱导梨指示植物 |
RT-PCR detection and sequence analysis of Pear blister canker viroid infecting pear |
|
| ZHU Hui,WANG Guo-ping,YANG Zuo-kun,WEN Li-hong,CHEN Jie,XU Wen-xing,HONG Ni. RT-PCR detection and sequence analysis of Pear blister canker viroid infecting pear[J]. Acta Phytopathologica Sinica, 2017, 47(4): 558-562 | |
| Authors: | ZHU Hui WANG Guo-ping YANG Zuo-kun WEN Li-hong CHEN Jie XU Wen-xing HONG Ni |
| Affiliation: | College of Plant Science and Technology, Key Lab of Crop Disease Monitoring and Safety Control in Hubei,Huazhong Agricultural University,Wuhan 430070,China; National Key Lab of Agromicrobiology,Huazhong Agricultural University,Wuhan 430070,China |
| Abstract: | Pear blister canker viroid (PBCVd) is a member of the genus Apscaviroid in the family Pospiviroidae.The viroid naturally infects pear (Pyrus spp.) and apple (Malus spp.). In this study, for the first time, PBCVd was detected in seven pear trees from three provinces of China by using RT-PCR. Except the isolate XH consisting of 314 nucleotides (nt), the complete RNA of other six PBCVd isolates consisted of 312 nt.These isolates shared 98.4%-100%identity with each other and 88.7%-100%identity with previously reported isolates at the nucleotide level. Phylogenetic analysis revealed that these isolates, together with a reference isolate P2098T, clustered into the same group. These results provide important information for further understanding the PBCVd occurrence in pear plants and enhancing the monitoring of PBCVd in China. |
| Keywords: | Pyrus spp. Pear blister canker viroid detection sequence analysis |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《植物病理学报》浏览原始摘要信息 | |
| 点击此处可从《植物病理学报》下载全文 | |