不同营养元素对开菲尔多糖产量的影响

开菲尔是一种由开菲尔粒发酵的功能性酒精性的乳制品.在开菲尔粒发酵培养期间,对外加营养元素进行单因素与正交试验,以研究其对开菲尔多糖产量的影响.结果表明:在无菌灭菌乳中添加3％麦芽糖、4％蔗糖、4％葡萄糖时,开菲尔多糖的产量最高,达到1.92 g/L;在无菌灭菌乳中添加酵母膏0.2％、蛋白胨1％、L-酪氨酸0.004％时,开菲尔多糖的产量最高,达到2.07 g/L;在无菌灭菌乳中添加0.4％ KH2PO4、0.08％ MgSO4·7H2O、0.02％CaCO3、0.25％NaC1时,开菲尔多糖的产量最高,达到2.01 g/L.

Effects of Different Nutrients on Polysaccharide Production during Kefir Fermentation

In this study, the production of polysaccharides by kefir grains during milk fermentation was optimized with respect to the amount of nutrients added to the fermentation medium （sterilized milk） employing one-factor-at-a-time and orthogonal array design methods. The results showed that the optimal carbon sources added to sterilized milk were 3% maltose, 4% sucrose and 4% glucose, resulting in a maximum yield of polysaccharides of 1.92 g/L, the optimal nitrogen sources were 0.2% yeast extract, 1% peptone and 0.004% L-tyrosine, resulting in a maximum yield of polysaccharides of 2.07 g/L, and the optimal inorganic salts were 0.4% KH2PO4, 0.08% MgSO4 ·7H2O, 0.02% CaCO3 and 0.25% NaCl, resulting in a maximum yield of polysaccharides of 2.01 g/L.