玉米密码子用法分析

生物中普遍存在密码子使用偏性,这对外源基因的表达强度有着较大影响。本研究运用Codon W软件和CUSP程序对玉米基因组中的7402个蛋白质编码基因序列进行了分析,计算了编码氨基酸的密码子使用频率,并将其与人、酵母、大肠杆菌、果蝇等不同种类的模式生物及与拟南芥、烟草、水稻、小麦等双、单子叶植物进行比较,结果显示玉米密码子偏爱性与模式生物有不同程度的差异,与拟南芥、烟草双子叶植物的密码子使用频率差异较大,与水稻、小麦单子叶植物的密码子偏爱性一致。分析结果对玉米基因的转化及高效表达系统的选择等具有重要指导意义。

ANALYSIS OF CODON USAGE IN MAIZE

It's widespread existence of codon usage bias in organism which has an important influence for expression intensity of exogenous gene.In this study,coding sequences of 7402 proteins were analyzed by CodonW and CUSP programs in maize genome,calculating frequency of codon usage which encoded amino acids and comparing with variety classes of mode organisms such as:human being,yeast,E.coli,Drosophila.These results had shown that codon bias of maize was difference in various degrees with these mode organisms.Comparing codon usage frequency of maize with dicotyledons and monocotyledons including Arabidopsis thaliana,tobacco,rice,wheat,the consequence indicated that codon usage frequency of maize was obviously different from Arabidopsis thaliana and tobacco which belonged to dicotyledon,but had a consistency with codon bias of rice and wheat which are monocotyl.These analytic results had important guidance for maize gene-transforming and selecting the high-efficient expression system.