| 应用IC-RT-PCR方法检测齿兰环斑病毒和建兰花叶病毒 |
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| 引用本文： | 李志勇,郭京泽,司贺龙,李兴红,董金皋. 应用IC-RT-PCR方法检测齿兰环斑病毒和建兰花叶病毒[J]. 河北农业大学学报, 2008, 31(6) |
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| 作者姓名： | 李志勇 郭京泽 司贺龙 李兴红 董金皋 |
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| 作者单位： | 北京农科院,植保环保所,北京,100089;河北农林科学院,谷子研究所,河北,石家庄,054000;天津出入境检验检疫局,天津,塘沽,300456;河北农业大学,植物保护学院,河北,保定,071001;北京农科院,植保环保所,北京,100089 |
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| 基金项目： | 北京市自然科学基金6052010 |
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| 摘 要： | 根据已报道的建兰花叶病毒和齿兰环斑病毒外壳蛋白基因序列设计PCR引物,建立了蝴蝶兰建兰花叶病毒和齿兰环斑病毒IC-RT-PCR检测方法。用该方法对天津地区8个蝴蝶兰标样检测,结果显示:8个标样中有4个检测到建兰花叶病毒,另外4个检测到齿兰环斑病毒。该方法简化了操作程序,为蝴蝶兰种苗病毒的快速检测奠定了基础。
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| 关 键 词： | 蝴蝶兰 齿兰环斑病毒 建兰花叶病毒 外壳蛋白基因 免疫捕获PCR 序列分析 |
The application of IC-RT-PCR in the detection of odontoglossum ringspot tobamovirus and cymbidium mosaic potexvirus |
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| LI Zhi-yong,GUO Jing-ze,SI He-long,LI Xing-hong,DONG Jin-gao. The application of IC-RT-PCR in the detection of odontoglossum ringspot tobamovirus and cymbidium mosaic potexvirus[J]. Journal of Agricultural University of Hebei, 2008, 31(6) |
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| Authors: | LI Zhi-yong GUO Jing-ze SI He-long LI Xing-hong DONG Jin-gao |
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| Affiliation: | LI Zhi-yong1,2,GUO Jing-ze3,SI Helong4,LI Xing-hong1,DONG Jin-gao4 |
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| Abstract: | Based on the reported odontoglossum ringspot Tobamovirus(ORSV) and cymbidium mosaic potexvirus(CymMV) coat protein sequence,two pairs of primer were designed and IC-RT-PCR method was developed.Eight samples were collected from Tianjing and IC-RT-PCR method was performed.Four samples were infected with ORSV and the others were infected with CymMV.The method provied a new approch for quickly detection of ORSV and CymMV. |
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| Keywords: | Phalaenopsis amabilis odontoglossum ringspot tobamovirus cymbidium mosaic potexvirus CP gene IC-RT-PCR sequence analysis |
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