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Sperm cryoconservation in Anarhichas sp., endangered cold-water aquaculture species with internal fertilization |
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| Authors: | N R Le François S G Lamarre H Tveiten P U Blier J Bailey |
| Institution: | (1) Ministère de l’agriculture, des pêcheries et de l’alimentation du Québec, Université du Québec à Rimouski/MAPAQ, Centre Aquacole Marin, 6, rue du Parc, Grande-Rivière, QC, Canada, G0C 1V0;(2) Laboratoire de biologie intégrative, Département de biologie, géographie et chimie, Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, QC, Canada, G5L 3A1;(3) Fiskeriforskning, Muninbakken 9-13, Breivika, Tromso, 9291, Norway;(4) Faculté des sciences de l’agriculture et de l’alimentation, Pavillon Paul-Comtois, Université Laval, Quebec, QC, Canada, G1K 7P4 |
| Abstract: | This study contributes to the identification of an adequate cryopreservation medium for wolffishes sperm (Anarhichas minor and A. lupus). Our results also confirm the presence of antifreeze proteins in the seminal fluid of the Atlantic wolffish (Anarhichas lupus) that could facilitate the process of cryoconservation in association with increased post-thaw motility and fertilization rates. Cryo-Fish, a commercial diluent was the most efficient in our trials, with 80% motility post-thaw. |
| Keywords: | Anarhichas Cryopreservation Internal fertilization Wolffish Antifreeze proteins |
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