| 苹果矮化砧GM256组织培养与快繁技术研究 |
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| 引用本文： | 郑亚杰,姚环宇. 苹果矮化砧GM256组织培养与快繁技术研究[J]. 吉林农业科学, 2008, 33(1): 26-27,32 |
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| 作者姓名： | 郑亚杰 姚环宇 |
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| 作者单位： | 吉林省农业科学院果树所,吉林,公主岭,136100;吉林省农业科学院果树所,吉林,公主岭,136100 |
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| 摘 要： | 通过对苹果矮化砧GM256的离体组织培养和快繁实验,探讨了GM256茎尖诱导、试管苗增殖、生根等组培技术。结果表明:GM256外植体能否诱导成苗,除了选择适宜的培养基外,必须要经过暗培养才能诱导出苗。适宜的增殖培养基为MS+3.0 mg/L BA+0.05 mg/L NAA,可使增殖率达到5.9%。暗培养对促进GM256试管苗的生根无明显效果,最佳生根培养基是1/2 MS+0.5 mg/L IBA。
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| 关 键 词： | 苹果矮化砧GM256 组织培养 快繁 |
| 文章编号： | 1003-8701（2008）01-0026-02 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007-04-20 |
Studies on Tissue Culture Technology of GM256,a Dwarf Rootstock of Apple |
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| ZHENG YA-jie,YAO Huan-yu. Studies on Tissue Culture Technology of GM256,a Dwarf Rootstock of Apple[J]. Journal of Jilin Agricultural Sciences, 2008, 33(1): 26-27,32 |
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| Authors: | ZHENG YA-jie YAO Huan-yu |
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| Abstract: | Experiment of in vitro Gm256,a dwarf rootstock of apple,was carried out to study its tissue culture techniques which included inducing of stem tips,propagation,rooting,etc.Results showed that except proper medium dark condition was also need for the explant of GM256 to be induced to a plantlet.The proper medium for propagation was MS 3.0 mg/L BA 0.05 mg/L NAA,and the propagation rate reached 5.9%.Culture in dark condition was of no use for rooting of GM256 plantlet.The proper medium for rooting was 1/2MS 0.5 mg/LBA. |
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| Keywords: | GM256 Rootstock of apple In vitro |
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