| 大豆种子发育阶段种皮细胞壁表面糖蛋白的变化 |
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| 引用本文： | 弭忠祥 赵小钒. 大豆种子发育阶段种皮细胞壁表面糖蛋白的变化[J]. 大豆科学, 1999, 18(1): 18-22 |
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| 作者姓名： | 弭忠祥 赵小钒 |
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| 作者单位： | [1]东北农业大学生命中心 [2]东北农业大学基础部 |
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| 摘 要： | 利用电镜细胞化学的方法，对大豆种子在发展的不同阶段，种皮细胞壁表面糖蛋白的变化进行了定位观察。经钌红标记染色，在种皮外层细胞壁的表面，糖蛋白呈现出较高电子密度，紧贴于细胞壁的表面，形成均匀的糖蛋白层。在种子发育的不同阶段，该糖蛋白层的厚度，随着种子扫育而不断增加，在开花后的第２５－３０天时，糖蛋白层的厚度达到最大，一般在５０－６０ｎｍ。种子在以后的发育过程中，糖蛋白层的厚度基本维持在这一水平。
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| 关 键 词： | 大豆 种子发育 糖蛋白 电镜 细胞化学 |
THE DISTRIBUTION OF GLYCOPROTEINS ON CELL WALL SURFACE OF SOYBEAN SEED COAT DURING DEVELOPMENTAL |
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| Mi Zhongxiang Lu Yin Hu Baozhong. THE DISTRIBUTION OF GLYCOPROTEINS ON CELL WALL SURFACE OF SOYBEAN SEED COAT DURING DEVELOPMENTAL[J]. Soybean Science, 1999, 18(1): 18-22 |
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| Authors: | Mi Zhongxiang Lu Yin Hu Baozhong |
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| Affiliation: | Mi Zhongxiang 1 Lu Yin 2 Hu Baozhong 1 |
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| Abstract: | Cytochemical localization of glycoproteins on cell wall surface of soybean seed coats during developmental was observed by electron microscopy. The glycoproteins layer was shown to be present on the cell wall surface after staining with ruthenium red. The thickness of glycoprotein layer seemed to have increased during seed development,and at stages of development 25-30 d. after anthesis the glycoproteins lays are thickest, about 50-60nm. |
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