| 白骨壤叶水提液清除自由基作用的研究 |
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| 引用本文： | 杨维,陈小雁,周浓,夏杏洲,韩维栋.白骨壤叶水提液清除自由基作用的研究[J].农产品加工．学刊,2009(12):27-29. |
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| 作者姓名： | 杨维 陈小雁 周浓 夏杏洲 韩维栋 |
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| 作者单位： | 1. 广东海洋大学,食品科技学院,广东,湛江,524088
2. 广东海洋大学,农学院,广东,湛江,524088 |
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| 基金项目： | 广东海洋大学红树林团队项目 |
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| 摘 要： | 研究了白骨壤叶的水浸提工艺,并采用邻苯三酚自氧化法、水杨酸法、DPPH法,分别测定了白骨壤叶水提液清除超氧阴离子自由基、羟自由基和DPPH自由基的能力。结果表明,水浸提白骨壤叶的最适工艺条件为:料液比1∶25(g∶mL),温度50℃,时间120min,提取率8.14%。白骨壤叶水提液对超氧阴离子(·O2)-、羟基自由基(·OH)及DPPH·自由基均有一定的清除活性,且与其浓度呈正相关,其IC50分别为1.16,1.42,2.27mg/mL,因此白骨壤叶水提液具有一定的体外抗氧化活性。
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| 关 键 词： | 白骨壤叶 超氧阴离子 羟自由基 DPPH自由基 |
Study on Free Radical Scavenging Activity of the Water Extracts from the Leaves of Avicennia Marina |
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| Yang Wei,Chen Xiaoyan,Zhou Nong,Xia Xingzhou,Han Weidong.Study on Free Radical Scavenging Activity of the Water Extracts from the Leaves of Avicennia Marina[J].Nongchanpin Jlagong.Xuekan,2009(12):27-29. |
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| Authors: | Yang Wei Chen Xiaoyan Zhou Nong Xia Xingzhou Han Weidong |
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| Abstract: | The technology of water extracts from the leaves of Avicennia Marina was studied in this paper, then the methods of pyrograllol auto- oxidation, salicylic acid, DPPH· were used to assay the scavenging quality of superoxide free radical (·O2)-,hydroxyl free radical (·OH),DPPH·free radical in the water extracts of Avicennia Marina. The results showed that the extraction rate goes up to 8.14% as the extraction proportion of solvent and stuff 1∶25 (g∶mL),extraction temperature 50 ℃,extraction time 120 min. The extracts showed certain scavenging effect on·O2-,·OH and DPPH·under the condition of experiment,and the scavenging effect were positively correlative with concentration. The IC50 on ·O2-,·OH and DPPH· were respectively 1.16 mg/mL,1.42 mg/mL and 2.27 mg/mL. So the water extracts of Avicennia Marina showed a certain extent antioxidant effect in vitro. |
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| Keywords: | avicennia marina superoxide anion hydroxyl radical DPPH· |
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