农杆菌介导三价融合基因Rirol转化八棱海棠的研究

研究了以口蹄疫病毒FMDV 2A序列融合多基因在八棱海棠遗传转化上的应用,将DREB,IRT1和rolC融合三价基因(Rirol)通过农杆菌介导法转化八棱海棠,获得了一批nptⅡ抗性植株,经报告基因检测、PCR和Southern杂交分析证明融合基因成功导入到八棱海棠中。对转基因八棱海棠相关性状分析表明,转基因八棱海棠比对照具有较强的耐盐性,在表型上,表现节间缩短,分枝性强,侧根发达,证明外源融合基因在转基因植株中得到表达。试验初步证明了利用FMDV 2A序列进行多基因转化八棱海棠的可行性,为苹果等果树多基因转化提供了新的思路和途径。

Agrobacterium-mediated transformation of Malus micromalus with trivalent genes Rirol

DREB,IRT1 and rolC genes were fused with FMDV 2A region and transformed to Malus micromalus mediated by Agrobacterium tumefaciens. Some kanamycin-resistant plants were obtained by selection. The fused genes were proved to integrate the genome of some Km-resistant plants of Malus micromalus by GUS staining,PCR and Southern bolting. The transgenic plants showed higher resistance to salt stress,shortened internodes and more branching of shoots and roots. The study primarily proved that co-transforming multiple genes fused by FMDV 2A region may be a new way of introducing multiple genes into fruit trees.