| 砂糖橘上碎叶病毒外壳蛋白基因的克隆及序列分析 |
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| 引用本文： | 孟祥春,向旭,钟云,刘岩,易干军. 砂糖橘上碎叶病毒外壳蛋白基因的克隆及序列分析[J]. 果树学报, 2007, 24(6): 796-798 |
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| 作者姓名： | 孟祥春 向旭 钟云 刘岩 易干军 |
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| 作者单位： | 广东省农业科学院果树研究所,广州,510640 |
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| 基金项目： | 广东省科技厅科技计划 , 广东省农业科学院科研基金 |
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| 摘 要： | 利用RT-PCR技术对广东砂糖橘碎叶病毒的外壳蛋白(CTLV-CP)基因进行了克隆和测序分析。DNA序列分析表明,砂糖橘CTLV-CP基因的cDNA序列全长为786bp,编码262个氨基酸。BLAST分析结果显示,所得序列与苹果、梨茎沟病毒(ASGV)外壳蛋白基因及其它柑橘CTLV-CP基因的同源性在87%～91%,证明所克隆的片段为CTLV-CP基因。
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| 关 键 词： | 砂糖橘 碎叶病毒(CTLV) 外壳蛋白(CP) 克隆 |
| 文章编号： | 1009-9980（2007）06-796-03 |
| 修稿时间： | 2007-05-13 |
Cloning and sequencing the coat protein gene of citrus tatter leaf virus from Shatangju mandarin cultivar |
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| MENG Xiang-chun,XIANG Xu,ZHONG Yun,LIU Yan,YI Gan-jun. Cloning and sequencing the coat protein gene of citrus tatter leaf virus from Shatangju mandarin cultivar[J]. Journal of Fruit Science, 2007, 24(6): 796-798 |
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| Authors: | MENG Xiang-chun XIANG Xu ZHONG Yun LIU Yan YI Gan-jun |
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| Affiliation: | Guangdong Fruit Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640 China |
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| Abstract: | The coat protein (CP) gene of citrus tatter leaf virus (CTLV) isolated from Shatangju mandarin cultivar were cloned and sequenced using RT-PCR technique. The results showed that the CTLV-CP cDNA was 786 bp,encoding protein of 262 amino acids. High identity (87% to 91%) was found between Shatangju mandarin cultivar CTLV-CP gene and ASGV CP gene isolated from apple,pear,and those CTLV-CP gene of many kinds of citrus plants,which proved that the cloned gene was that of CTLV-CP. |
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| Keywords: | Shatangju mandarin Citrus tatter leaf virus (CTLV) Coat protein (CP) Cloning |
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