CaDREB3启动子分离及其缺失体转基因烟草的获得

CaDREB3是辣椒DREB家族中1个成员,为明确CaDREB3受逆境胁迫诱导表达的分子机制,从辣椒基因组DNA中分离获得了CaDREB3上游的启动子,利用生物信息学软件进行顺式作用元件的分析,结果表明该启动子的TATA框为起始密码子ATG上游-89 bp至-86 bp的TATA,同时含有多种与逆境胁迫相关的顺式作用元件,如ABRE、HSE、MYB和TCA等,并使用Gateway技术构建了启动子6个缺失体的GUS融合载体,获得了6个缺失体的烟草转基因植株,这些结果为将来分析该启动子应答逆境胁迫的调控区域奠定了一定的基础。

Isolation of Promoter and Acquisition of Transgenic Tobacco Plants for Its 5' Deletion Mutation Analysis

CaDREB3 is a member of the DREB family in pepper. In order to further clarify the molecular mechanism of the stress-induced expression of CaDREB3, we isolated the upstream promoter of CaDREB3 from pepper genomic DNA. Bioinformatics analysis illustrated that the TATA box located from -89 bp to -86 bp, upstream from start codon(ATG), and a variety of stress-associated cis-acting elements were included, such as ABRE, HSE, MYB and TCA, etc. Five deletions fused to GUS--reported gene of CaDREB3 promoter were constructed via Gateway technology and transgenic plants of the six deletions were generated. Taken together, the results above lay the foundation to determine the regulatory region of the promoter response to stress.