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雏鸭人工感染黄曲霉毒素及其毒素含量的动态变化
引用本文:刘艳丽,程安春,汪铭书,胡骑,陈孝跃. 雏鸭人工感染黄曲霉毒素及其毒素含量的动态变化[J]. 中国家禽, 2007, 29(17): 8-10
作者姓名:刘艳丽  程安春  汪铭书  胡骑  陈孝跃
作者单位:四川农业大学动物医学院禽病防治研究中心,四川雅安,625014;动物疫病与人类健康四川省重点实验室,四川雅安,625014
基金项目:国家科技攻关项目;教育部跨世纪优秀人才培养计划;四川省重点学科建设项目
摘    要:通过7日龄樱桃谷鸭饲喂黄曲霉毒素(AFB1)含量为150 bbp的全价饲料,复制雏鸭感染黄曲霉毒素急性中毒病例,观察中毒鸭的临床症状;于6、12、24、48、72、96、120、144、168和192 h分别剖杀2只,观察大体病理变化,并用ELISA试剂盒测定血液、肝脏中的AFB1含量.黄曲霉毒素急性中毒病例的临床症状主要为:生长停滞,跛行,运动失调,死前呈角弓反张等神经症状.剖检病理变化主要为:气囊混浊有黄色纤维素样渗出物,肝脏肿大呈土黄色,质地变脆并有出血点.饲喂黄曲霉毒素饲料6 h后即可从血液、肝脏中检出AFB1,随着饲喂黄曲霉毒素饲料时间的延长,血液、肝脏中AFB1的含量逐渐增高,7d时肝脏和血清中的AFB1分别高达53.4 ng/g和35.68 ng/g.

关 键 词:黄曲霉毒素  雏鸭
修稿时间:2007-04-04

Experimental Acute Poisoning of Aflatoxin and the Content Changes in Duckling
Liu Yanli,Cheng Anchun,Wang Mingshu,Hu Qi,Chen Xiaoyue. Experimental Acute Poisoning of Aflatoxin and the Content Changes in Duckling[J]. China Poultry, 2007, 29(17): 8-10
Authors:Liu Yanli  Cheng Anchun  Wang Mingshu  Hu Qi  Chen Xiaoyue
Abstract:Seven-day-old Cherry Valley ducklings were fed with the diet containing 150 bbp aflatoxin(AFB1) for observing clinical symptom of acute aflatoxin poisoning. Two ducklings were dissected at 6,12,24,48,72,96,120,144,168,192 hours after feeding to study the macroscopic pathological changes,and the content of AFB1 in blood and liver was detected by ELISA. The clinical symptoms was mainly that growth stagnated,lamely movement disorders,opisthotonos before death and other neurological symptoms. Macroscopic characteristic was that much yellow fibrin exuded from air cell, liver was swollen. AFB1 in liver and blood could be detected after feeding 6 hours. With the feeding time prolonged,the amount of AFB1 in blood and liver increased constantly. The amount of AFB1 in liver and blood was 53.4 ng/g and 35.68 ng/g at 7 day after feeding.
Keywords:ELISA
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