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| 2株鸽源新城疫病毒的毒力鉴定和基因型分析 | |
| 引用本文： | 高以明,张敬友,王水明,柯家法,李超美,朱雪良,张扬,严继宁,黄立业,任晓进,吴艳涛,刘文博.2株鸽源新城疫病毒的毒力鉴定和基因型分析[J].中国动物检疫,2011,28(7):36-39. |
| 作者姓名： | 高以明 张敬友 王水明 柯家法 李超美 朱雪良 张扬 严继宁 黄立业 任晓进 吴艳涛 刘文博 |
| 作者单位： | 1. 南京农业大学,江苏南京,210095 2. 江苏出入境检验检疫局,江苏南京,210001 3. 南京出入境检验检疫局,江苏南京,210001 4. 扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州,225009 |
| 基金项目： | 江苏检验检疫局科技计划项目，江苏省高校自然科学基础研究面上项目，江苏省教育厅指导计划项目 |
| 摘 要： | 2008年从临床鸽病例中分离到2株新城疫病毒毒株,经测定,鸡胚平均死亡时间(MDT)分别为48h和46h,8周龄SPF鸡静脉接种指数(IVPI)分别为2.88和2.91,一日龄雏鸡脑内接种指数(IPCI)分别为1.88和1.89,按照OIE推荐的标准均为强毒株。RT-PCR扩增出融合蛋白(F)基因片段,测序,推导的氨基酸序列中蛋白酶裂解位点附近的氨基酸序列均符合强毒特征序列。根据F蛋白的部分基因序列绘制的系统进化发生树和F基因片段中3种限制性内切酶(RE)位点分布判定这2个毒株均为基因Ⅶc型。 |
| 关 键 词： | 鸽 新城疫病毒 毒力 遗传发生树 基因型 |
Phylogenetic Analysis and Virulence Identification of Two Newcastle Disease Virus Isolates from Pigeons |
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| Gao Yingming,Zhang Jingyou,Wang Shuiming,Ke Jiaf,Li Chaomei,Zhu Xueliang,Zhang Yang,Yan Ji ning,Huang Liye,Ren Xiaojin,Wu Yantao,Liu Wentao.Phylogenetic Analysis and Virulence Identification of Two Newcastle Disease Virus Isolates from Pigeons[J].China Journal Of Animal Quarantine,2011,28(7):36-39. | |
| Authors: | Gao Yingming Zhang Jingyou Wang Shuiming Ke Jiaf Li Chaomei Zhu Xueliang Zhang Yang Yan Ji ning Huang Liye Ren Xiaojin Wu Yantao Liu Wentao |
| Institution: | Gao Yiming1,2,Zhang Jingyou3,Wang Shuiming2,Ke Jiafa3,Li Chaomei2,Zhu Xueliang2,Zhang Yang3,Yan Jining2,Huang Liye2,Ren Xiaojin2,Wu Yantao4,Liu Wenbo4(1.Nanjing Agricultural University,Nanjing 210095,China,2.Nanjing Entry-Exit Inspection and Quarantine Bureau,Nanjing,210001,3.Jiangsu Entry Exit Inspection and Quarantine Bureau,4.MOA Key Lab for Animal Infectious Disease,Yangzhou Agricultural University,Yangzhou,Jiangsu 225009,China) |
| Abstract: | Two Newcastle disease virus isolates were got from dead pigeons in 2008.The virulence of the isolates were identified based on the MDT, ICPI and IVPI. The genes of fusion protein were sequenced.The amino acid sequence between 112 to 117 were l12R （K）RQKR Fll7.The restriction enzyme cleavage site mapping of the fusion protein gene between nucleotides 334 and 1682 and by sequence analysis between nucleotides 47 and 420.Both methods revealed the two isolates belong to genotype Vile.The characters of the isolates were as same as other isolates of this genotype from geese and chickens in recent years.It was shown that the virulence and genotype of the Newcastle disease virus from pigeons had changed. |
| Keywords: | Newcastle disease virus fusion protein gene virulence phylogenetic tree genotype |
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