| PCR法检测耐热菌模板制备方法研究 |
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| 引用本文： | 冯再平,仇农学,李建科. PCR法检测耐热菌模板制备方法研究[J]. 中兽医医药杂志, 2006, 25(4): 5-6 |
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| 作者姓名： | 冯再平 仇农学 李建科 |
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| 作者单位： | 1. 兰州理工大学生命科学与工程学院,甘肃,兰州,730050
2. 陕西师范大学食品工程系 |
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| 基金项目： | 兰州理工大学校科研和教改项目 |
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| 摘 要： | 以耐热菌为研究对象．比较了CTAB法、CTAB改进法、经典酶法、氯化苄法、SDS法等5种楼板制备方法．提取DNA直接电泳结果和PCR结果表明．经典酶法和氯化苄法均能提供较好质与量的DNA模板；综合考虑时间等因素选择氯化苄法为此5种模板制备方法中的最优。
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| 关 键 词： | 耐热菌 模板制备 |
| 文章编号： | 1000-6354（2006）04-0005-02 |
| 收稿时间： | 2006-02-17 |
| 修稿时间： | 2006-02-17 |
Sdudy on the preparation methods of templates in detection of Alicyclobacillus acidoterrestris by PCR |
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| FENG Zai-ping,QIU Nong-xue,LI Jian-ke. Sdudy on the preparation methods of templates in detection of Alicyclobacillus acidoterrestris by PCR[J]. Journal of Traditional Chinese Veterinary Medicine, 2006, 25(4): 5-6 |
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| Authors: | FENG Zai-ping QIU Nong-xue LI Jian-ke |
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| Affiliation: | College of Life Science and Engincering,Lanzhou University of Technology,Lanzhou Gansu 730050,China |
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| Abstract: | Five different DNA extraction methods,sueb as the CTAB,the improved CTAB,the enzyme method,the benzyl chloride method and the SDS method,were evaluated for their effeot in preparing PCR templates from the Alicyclobucillus acidoterrestris.The results of DNA electrophoresis and PCR showed that both methods of benzyl chloride and enzyme could obtain DNA samples with sufficient quantity and superior quality,But considering the enyme method spending more time,the benzyl chloride method is more efficient |
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| Keywords: | PCR |
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