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甜瓜种子醇溶蛋白的反相HPLC分析鉴定
引用本文:王强,阮晓,陈贺,孙晓路,盛建东,颜启传. 甜瓜种子醇溶蛋白的反相HPLC分析鉴定[J]. 作物学报, 2005, 31(2): 177-182
作者姓名:王强  阮晓  陈贺  孙晓路  盛建东  颜启传
作者单位:浙江大学宁波理工学院,生物与制药工程系,浙江宁波 315100
基金项目:国家自然科学基金,浙江大学校科研和教改项目,新疆科技厅资助项目
摘    要:针对目前国内甜瓜的主栽品种K96-8×6-1、T-5Xred1-28和94×8-1的自交系及杂交种种子醇溶贮藏蛋白进行反相HPLC法分离。结果表明其色谱图明显不同,可以用于该品种(系)的鉴定,认为RP-HPLC技术是一种快速、准确、可信的甜瓜品种鉴定手段。

关 键 词:甜瓜  品种鉴定  反相高效液相色谱  
收稿时间:2003-11-28
修稿时间:2004-04-04

Melon (Cucumis melo L.) Cultivar Identification by Reversed-phase High Performance Liquid Chromatography of Seed Proteins
WANG Qiang,RUAN Xiao,CHEN He,SUN XiaoLu,SHENG JianDong,YAN QiChuan. Melon (Cucumis melo L.) Cultivar Identification by Reversed-phase High Performance Liquid Chromatography of Seed Proteins[J]. Acta Agronomica Sinica, 2005, 31(2): 177-182
Authors:WANG Qiang  RUAN Xiao  CHEN He  SUN XiaoLu  SHENG JianDong  YAN QiChuan
Affiliation:Department of Biological and Pharmaceutical Engineering, Zhejiang University, Ningbo Institute of Technology, Ningbo 315100, Zhejiang
Abstract:Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) of cereal grain proteins has been receiving much attention in the agricultural literature published in recent years. A study of melon (Cucumis melo L.) hybrid parents S ♂ and S ♀ and F1 hybrids of K96-8×6-1, T-5×red1-28 and 94×8-1 was undertaken to determine if RP-HPLC could be adapted to the cultivar identification of melons. Chromatograms of the three inbred lines and F1 hybrids of cultivars were shown to be different and were used to characterize those cultivars. RP-HPLC was shown to be a quick, replicable and reliable method of melon cultivar identification for general screening of seed lots.
Keywords:Melon(Cucumis melo L.)  Cultivar identification  Reversed-Phase High Performance Liquid Chromatography
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