泰乐菌素高效降解菌的筛选及降解特性研究

发酵法生产泰乐菌素过程中会产生大量药渣,因残留抗生素的存在,极大地限制了其资源化利用。本研究采用微生物法降解药渣中残留泰乐菌素。结果表明：从堆放泰乐菌素药渣附近土壤中分离筛选到1株高效降解药渣泰乐菌素的菌株,经16S rDNA鉴定为无丙二酸柠檬酸杆菌（Citrobacter amalonaticus）。该菌株适宜生长pH值为6.0～7.0、温度为30～35°C。在30°C、pH6.5条件下,将无丙二酸柠檬酸杆菌按质量比为10%的量接种于含50 mg.L-1泰乐菌素培养基中,经48 h发酵处理后,95.2%的泰乐菌素被降解。提示利用微生物法可有效降解药渣中残留泰乐菌素。

Isolation and Degradation Characteristics of a Tylosin-degrading Strain

A mass of pharmaceutical waste is produced during tylosin production by fermentation.The resource utilization of pharmaceutical waste is restricted greatly because of the existence of antibiotic residue.The aim of this study was to investigate the degradation of tylosin residue in pharmaceutical waste by microbiological method.The results showed that a tylosin-degrading strain was isolated from the soil on which the tylosin pharmaceutical waste was long stacked.This strain was identified by 16S rDNA as Citrobacter amalonaticus.The optimal growth conditions of C.amalonaticus were at pH6.0~7.0 and temperature 30~35°C.The amount of 95.2 % of tylosin in medium was degraded by C.amalonaticus after fermentation for 48 h with conditions of initial bacteria concentration 10%,temperature 30°C,and pH 6.5.These indicate that tylosin residue in pharmaceutical waste can been degraded by using microorganism.