北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化(摘要)(英文)

目的]研究北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化。方法]从156株北冰洋海洋细菌中筛选出淀粉酶高产菌株ArcB84A,并对其进行了菌株形态学鉴定、16SrRNA分子鉴定以及产酶条件优化。结果]菌株ArcB84A属于交替假单胞菌属。菌株B84A的最佳产酶条件为:培养基起始pH值为7.0-8.0;最佳碳源为5‰葡萄糖;最佳氮源为5‰蛋白胨;TritonX-100、Tween20、Tween80等表面活性剂可以提高菌株淀粉酶活性,其中10‰Tween80的效果最为明显。结论]该研究为海洋细菌淀粉酶的生产与应用提供了理论依据。

Screening and Identification of Amylase-producing Strain from Arctic Ocean and Optimization of Enzyme Producing Conditions

Objective] The aims were to investigate the screening and identification of amylase-producing marine bacteria from Arctic sea and the optimization of the amylase producing conditions. Method] A high-yield strain for producing amylase named ArcB84A was isolated from a total of 156 marine bacteria of Arctic sea. Then,the morphological identification of the strain,molecular identification of 16S rRNA and optimization of fermentation conditions were conducted. Result] ArcB84A strain was a member of Pseudoalteromonas genus. The optimum conditions for enzyme production of B84A strain included that,the initial pH value of the medium was 7.0-8.0,and the best carbon and nitrogen sources respectively were 5‰ glucose and peptone. Surfactants including TritonX-100,Tween20 and Tween80 could increase amylase activity of the strain,in which,the effect of 10‰ Tween80 was the most obvious.