东北矮紫杉细胞悬浮培养中褐化问题的研究(英文)

目的]研究东北矮紫杉悬浮培养细胞褐化现象,为紫衫细胞悬浮培养提供指导方法。方法]以东北矮紫杉愈伤组织为试验材料,探讨不同培养基、N/P 比、pH、培养转速、光照及强度等因素对矮紫杉细胞悬浮培养褐化的影响。结果]将无褐化的愈伤组织转接到pH7.0的2MB5培养基中,于22℃、1500lx光强、90r/min 转速的条件下光照培养24h,细胞褐化现象能得到明显抑制。结论]该研究结果为紫衫细胞悬浮培养奠定了基础,对于大规模工业化生产紫杉醇具有重要意义。

Study on the Browning in Cell Suspension Culture of Taxus cuspidata

Objective] This study aimed to investigate the browning of T.cuspidata cells in suspension culture and provide the guidance for the cell suspension culture of T.cuspidata.Method] T.cuspidata callus was used as experimental materials,to explore the effect of different medium,N/P ratio,pH,shaking speed,illumination time and light intensity and other factors on browning of T.cuspidata cells in suspension culture.Result] Non-browning callus was transferred to 2MB5 medium(pH 7.0) for illumination culture at 22 ℃ under light intensity of 1 500 lx with shaking speed of 90 r/min for 24 h.Results showed that the cell browning was significantly inhibited.Conclusion] This study laid the foundation for cell suspension culture of T.cuspidata and had important significance to the large-scale industrial production of paclitaxel.