三倍体九孔鲍的育种和养成技术研究

当 5 0 %九孔鲍受精卵出现第一极体后 ,以 6 -DMAP为诱导剂 ,用不同的浓度和不同的持续时间抑制第二极体的释放诱导三倍体 ;并进行了生产性苗种培育和养成试验。结果表明 ,6 -DMAP的诱导浓度为30 0μmol·dm-3 、诱导持续时间为 10min时 ,其胚胎的三倍体率为 90 %以上 ,并且胚胎的孵化率高达 85 % ,幼体的畸形率较低 ,为 5 0 %～ 5 5 % ;经过 7个月培育 ,试验组养成鲍的三倍体率为 6 5 %以上 ;试验组养成鲍的平均壳长比对照组增长 10 % ,平均体重比对照组增重 30 %。与二倍体鲍相比较 ,三倍体鲍在壳长增长和体重增重等方面 ,显示了显著的优势 t>t0 .0 1( 58) ]。

Seed breeding and culturing of triploid abalone Haliotis diversicolor aquatilis

When 50% of zygotes of Haliotis diversicolor aquatilis develop polar body . , with 6- DMAP as derivat ive , various concentration and dif ferent treatment time are used to induce triplo id by inhibiting releasing of the second polar body ò ; the experiment was carried out for productive seed breeding and culturing. Results show that when 6- DMAP is at the concentration of 300 Lmol#dm - 3 , and treatment time is 10min, the triploid rate of embryo is above 90%; In addition, embryonic hatching rate can reach as high as 85%, with larval deformity rate as low as 50%- 55% . After 7 months of cultivation, the rate of triploid of the culture abalone in test group is above 65%. On-the-spot acceptance check by experts proves that the average length of the shell of the culture abalone in test group grew 10% longer than that of control group. The average weight of the culture abalone in test group grew 30% weightier than that of the control group. Compared with diploid control group, triploid abalone displayed notable advantages in the growth of such aspect s as shell length and body weight ( t> t 0. 01( 58) ) .