| 野大麦幼根和幼叶悬浮细胞系的原生质体培养 |
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| 引用本文： | 程肖蕊,张亚兰,杨松涛.野大麦幼根和幼叶悬浮细胞系的原生质体培养[J].西北农林科技大学学报(社会科学版),1998,26(2):22-26. |
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| 作者姓名： | 程肖蕊 张亚兰 杨松涛 |
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| 作者单位： | 解放军农牧大学生物教研室 |
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| 摘 要： | 以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ（Ｔｒｉｎ．）Ｌｉｎｋ）的幼根、幼叶为外植体，诱导愈伤组织，建立悬浮细胞系。利用胚性悬浮细胞系进行质壁分离处理再游离原生质体，在不同的光照条件下进行原生质体培养，并由原生质体再生出小细胞团。
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| 关 键 词： | 野大麦 原生质体 细胞分裂 |
Protoplast Culture from Suspension Cell Line of Young Root and Leaf of Wild Barley (Hordeum brevisubulatum(Trin.) Link) |
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| Cheng Xiaorui,Zhang Yalan,Yang Songtao,Li Yanfang.Protoplast Culture from Suspension Cell Line of Young Root and Leaf of Wild Barley (Hordeum brevisubulatum(Trin.) Link)[J].Journal of Northwest Sci-Tech Univ of Agr and,1998,26(2):22-26. |
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| Authors: | Cheng Xiaorui Zhang Yalan Yang Songtao Li Yanfang |
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| Institution: | Feng Jiatao Yang Jianguo (College of Water Conservancy and Architectural Engineering,Northwestern Agricultural University,Yangling,Shaanxi,712100) |
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| Abstract: | In this study,young roots and young leaves of wild barley ( Hordeum brevisubulatum (Trin.) Link) were used as explants to induce calli.Through several subcultures,embryogenic calli were selected and adopted to establish suspension cell lines.Protoplasts were isolated from embryogenic suspension cell line after plasmolysis treatment and cultured under different light conditions.Cell mini clusters regenerated from protoplasts. |
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| Keywords: | wild barley ( Hordeum brevisubulatum (Trin ) Link) protoplast cell division |
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